Production of recombinant proteins in transgenic plants: practical considerations

AR Kusnadi, ZL Nikolov… - Biotechnology and …, 1997 - Wiley Online Library
This review is based on our recent experience in producing the first commercial recombinant
proteins in transgenic plants. We bring forward the issues that have to be considered in the …

Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification

EE Hood, DR Witcher, S Maddock, T Meyer… - Molecular …, 1997 - Springer
We have produced in transgenic maize seed the glycoprotein, avidin, which is native to
avian, reptilian, and amphibian egg white. A transformant showing high-level expression of …

Molecular farming of industrial proteins from transgenic maize

EE Hood, A Kusnadi, Z Nikolov, JA Howard - Chemicals via higher plant …, 1999 - Springer
Recombinant egg white avidin and bacterial B-glucuronidase (GUS) from transgenic maize
have been commercially produced. High levels of expression were obtained in seed by …

Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants

DR Witcher, EE Hood, D Peterson, M Bailey, D Bond… - Molecular …, 1998 - Springer
We have generated transgenic maize seed containing β-glucuronidase (GUS) for
commercial production. While many other investigators have demonstrated the expression …

[HTML][HTML] The hsp90 co-chaperone XAP2 alters importin β recognition of the bipartite nuclear localization signal of the Ah receptor and represses transcriptional activity

JR Petrulis, A Kusnadi, P Ramadoss… - Journal of Biological …, 2003 - ASBMB
The mouse aryl hydrocarbon receptor (mAhR) is a ligand-activated transcription factor that
exists in a tetrameric, core complex with a dimer of the 90-kDa heat shock protein, and the …

[HTML][HTML] Mechanistic insights into the events that lead to synergistic induction of interleukin 6 transcription upon activation of the aryl hydrocarbon receptor and …

BC DiNatale, JC Schroeder, LJ Francey… - Journal of biological …, 2010 - ASBMB
The aryl hydrocarbon receptor (AHR) is the ligand-activated transcription factor responsible
for mediating the toxicological effects of dioxin and xenobiotic metabolism. However, recent …

Process and economic evaluation of the extraction and purification of recombinant β‐glucuronidase from transgenic corn

RL Evangelista, AR Kusnadi, JA Howard… - Biotechnology …, 1998 - Wiley Online Library
A process model for the recovery and purification of recombinant β‐glucuronidase (rGUS)
from transgenic corn was developed, and the process economics were estimated. The base …

Production and purification of two recombinant proteins from transgenic corn

AR Kusnadi, EE Hood, DR Witcher… - Biotechnology …, 1998 - Wiley Online Library
This study reports the production, purification, and characterization of recombinant
Escherichia coli β‐glucuronidase (GUS) and chicken egg‐white avidin from transgenic corn …

[HTML][HTML] Ah receptor represses acute-phase response gene expression without binding to its cognate response element

RD Patel, IA Murray, CA Flaveny, A Kusnadi… - Laboratory …, 2009 - Elsevier
Repression of the nuclear factor-κB (NF-κB) pathway has been extensively researched
because of its pivotal role in inflammation. We investigated the potential of the aryl …

Recombinant aprotinin produced in transgenic corn seed: extraction and purification studies

AR Azzoni, AR Kusnadi, EA Miranda… - Biotechnology and …, 2002 - Wiley Online Library
Expression in transgenic plants is potentially one of the most economical systems for large‐
scale production of valuable peptide and protein products. However, the downstream …