Molecular characterization of the human interleukin (IL)-17 receptor

Cytokine. 1997 Nov;9(11):794-800. doi: 10.1006/cyto.1997.0240.

Abstract

Human interleukin 17 (hIL-17) is a T-cell derived cytokine that exhibits 63% amino acid sequence identity to mouse IL-17 (mIL-17) and 57% identity to a viral protein encoded by the herpesvirus saimiri (HSV) gene 13 (HVS13). The IL-17 family of proteins binds to a unique mouse receptor (mIL-17R). Using nucleic acid hybridization techniques, a cDNA encoding a human homologue of the mIL-17R (hIL-17R) was isolated from a human T cell library. The predicted amino acid sequence of the hIL-17R is 69% identical to the mIL-17R, shares no homology with previously identified cytokine receptor families, and exhibits a broad tissue distribution. The hIL-17R gene was localized to chromosome 22. Monoclonal antibodies (mAbs) generated against the hIL-17R were able to block the IL-17-induced production of cytokine from human foreskin fibroblast (HFF) cells. Binding studies suggest that recombinant hIL-17 binds to the hIL-17R with low affinity.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte / chemistry
  • Cell Separation
  • Chromosome Mapping
  • Chromosomes, Human, Pair 22*
  • Electrophoresis, Polyacrylamide Gel
  • Flow Cytometry
  • Humans
  • Interleukin-17
  • Interleukin-6 / biosynthesis
  • Interleukins / antagonists & inhibitors
  • Interleukins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • RNA, Messenger / metabolism
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin / immunology
  • Receptors, Interleukin / metabolism
  • Receptors, Interleukin-17
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • T-Lymphocytes / chemistry
  • Tissue Distribution

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • IL17RA protein, human
  • Il17a protein, mouse
  • Il17ra protein, mouse
  • Interleukin-17
  • Interleukin-6
  • Interleukins
  • RNA, Messenger
  • Receptors, Interleukin
  • Receptors, Interleukin-17
  • Recombinant Proteins

Associated data

  • GENBANK/U58917