Abstract
Intercellular adhesion molecules play an important role in the generation of T lymphocyte-mediated immune responses. Here, we describe a novel accessory molecule, DNAX accessory molecule-1 (DNAM-1), that is constitutively expressed on the majority of peripheral blood T lymphocytes. DNAM-1 is a 65 kDa transmembrane glycoprotein consisting of 318 aa including two immunoglobulin-like domains. Anti-DNAM-1 monoclonal antibody (MAb) inhibits T and NK cell-mediated cytotoxicity against a variety of tumor cell targets and blocks cytokine production by alloantigen-specific T cells. In addition, DNAM-1 is a tyrosine-phosphorylated signal-transducing molecule that participates in primary adhesion during cytotoxic T lymphocyte (CTL)-mediated cytotoxicity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Differentiation, T-Lymphocyte*
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Antigens, Surface / blood
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Antigens, Surface / immunology
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Antigens, Surface / isolation & purification
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B-Lymphocytes / chemistry
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Base Sequence
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Cell Adhesion Molecules / blood
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Cell Adhesion Molecules / immunology*
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Cell Adhesion Molecules / isolation & purification
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Cloning, Molecular
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Cytotoxicity, Immunologic*
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DNA, Complementary / isolation & purification
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Epitopes / immunology
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Humans
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Killer Cells, Natural / immunology
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Lymphokines / metabolism
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Membrane Proteins / blood
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Membrane Proteins / immunology*
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Membrane Proteins / isolation & purification
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Mice
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Molecular Sequence Data
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Monocytes / chemistry
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Signal Transduction / immunology
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T-Lymphocytes, Cytotoxic / chemistry
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Cytotoxic / metabolism
Substances
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Antigens, Differentiation, T-Lymphocyte
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Antigens, Surface
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CD226 antigen
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Cell Adhesion Molecules
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DNA, Complementary
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Epitopes
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Lymphokines
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Membrane Proteins