Abstract
This study was undertaken to determine the effects of adrenomedullin (AM) on the secretion of cytokine-induced neutrophil chemoattractant (CINC), a member of the interleukin-8 family, from lipopolysaccharide-stimulated rat alveolar macrophages in vitro. AM significantly increased cAMP levels in alveolar macrophages in a dose-dependent fashion. On the other hand, AM significantly inhibited CINC secretion from alveolar macrophages in a dose-dependent fashion, and 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) also significantly inhibited CINC secretion. These findings suggest that AM may play important roles in the regulation of airway inflammation via a cAMP-dependent mechanism.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Adrenomedullin
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Animals
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Bronchodilator Agents / pharmacology*
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Cells, Cultured
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Chemokines, CXC*
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Chemotactic Factors / classification
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Chemotactic Factors / metabolism*
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Cyclic AMP / analysis
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Dose-Response Relationship, Drug
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Growth Substances / classification
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Growth Substances / metabolism*
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Intercellular Signaling Peptides and Proteins*
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Interleukin-8 / classification
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Lipopolysaccharides / pharmacology
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Macrophages, Alveolar / drug effects*
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Male
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Peptides / pharmacology*
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Rats
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Rats, Wistar
Substances
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Bronchodilator Agents
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Chemokines, CXC
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Chemotactic Factors
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-8
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Lipopolysaccharides
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Peptides
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Adrenomedullin
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8-Bromo Cyclic Adenosine Monophosphate
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Cyclic AMP