Abstract
A solid-phase enzyme-linked immunosorbent assay has been established using a cloned fusion protein, CtermCENP-B [beta-gal], as antigen. The fusion protein carries the major epitope of CENP-B, the major centromeric autoantigen. The enzyme-linked immunosorbent assay was more sensitive than immunofluorescence techniques in detecting anticentromere antibodies in patients with scleroderma or Raynaud's disease, and was weakly positive in 3% of normal controls and in 3% of 70 patients with other connective tissue diseases.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Aged
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Autoantibodies / analysis*
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Autoantigens / immunology*
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Centromere / immunology*
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Centromere Protein A
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Chromosomal Proteins, Non-Histone*
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Chromosomes / immunology*
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Cloning, Molecular
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Connective Tissue Diseases / immunology
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Enzyme-Linked Immunosorbent Assay
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Humans
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Male
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Middle Aged
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Nucleoproteins / immunology*
Substances
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Autoantibodies
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Autoantigens
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Centromere Protein A
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Chromosomal Proteins, Non-Histone
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Nucleoproteins