[Effect of low-power laser irradiation on the structure and properties of protein molecules under non-specific energy absorbance]

E O Romodanova; T S Diubko; T F Morozova; O D Roshal'; V B Distanov; Iu O Hurkalenko

[Effect of low-power laser irradiation on the structure and properties of protein molecules under non-specific energy absorbance]

Fiziol Zh (1994). 2003;49(2):96-9.

[Article in Ukrainian]

Authors

E O Romodanova¹, T S Diubko, T F Morozova, O D Roshal', V B Distanov, Iu O Hurkalenko

Affiliation

¹ Laser Biology and Laser Medicine Research Iinstitute, V.N. Karazin Kharkov National University.

PMID: 12945122

Abstract

The influence of the helium-neon laser emission on HSA was investigated at present work by means of the fluorescent probe MNBIS (4-morpholino-7-oxy-7-H-benzo-[de]-benzo-[4,5]-imidazo-[2,1-a]- izoquinolin-5-sulfonic acid) sensitive to protein conformational changes using. Fluorescence spectra parameters of the probe and kinetics of probe interaction with protein molecules were analyzed. Possible mechanisms of the helium-neon laser irradiation on water-protein solution structure are discussed.

Publication types

English Abstract

MeSH terms

Fluorescent Dyes / chemistry
Fluorescent Dyes / radiation effects
Helium
Humans
Hydrogen-Ion Concentration
Kinetics
Lasers*
Molecular Structure
Neon
Protein Binding
Protein Conformation
Proteins / chemistry*
Proteins / radiation effects*
Radiation Dosage
Serum Albumin / chemistry
Serum Albumin / radiation effects
Solutions / chemistry
Spectrometry, Fluorescence
Tryptophan / chemistry
Tyrosine / chemistry
Water / chemistry

Substances

Fluorescent Dyes
Proteins
Serum Albumin
Solutions
Water
Helium
Tyrosine
Neon
Tryptophan