Abstract
We investigated the effect of TGF-beta1 on liver metastasis of pancreatic cancer using surgical specimens of pancreatic cancer and human pancreatic cancer cell lines Capan-2 and SW1990. Immunostaining of TGF-beta1 showed that TGF-beta1 positivity was significantly related to venous invasion and tumor staging, and also relatively associated with liver metastasis. Cellular invasion and protease production of MMP-2 and u-PA, and in vivo liver metastasis were significantly enhanced after treatment of cells with TGF-beta1. These findings suggest that TGF-beta1 might play an important role in enhancing liver metastasis of pancreatic cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / metabolism
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Adenocarcinoma / secondary*
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Adult
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Aged
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Aged, 80 and over
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Animals
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Female
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Humans
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Immunoenzyme Techniques
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Liver Neoplasms / metabolism
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Liver Neoplasms / secondary*
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Male
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Matrix Metalloproteinases / metabolism
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Staging
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Neoplasm Transplantation
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Pancreatic Neoplasms / metabolism
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Pancreatic Neoplasms / pathology*
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Pancreaticoduodenectomy
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Transforming Growth Factor beta / metabolism
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Transforming Growth Factor beta / pharmacology*
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Tumor Cells, Cultured / drug effects*
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Tumor Cells, Cultured / metabolism
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Urokinase-Type Plasminogen Activator / metabolism
Substances
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Transforming Growth Factor beta
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Urokinase-Type Plasminogen Activator
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Matrix Metalloproteinases