Abstract
This study examined the release of several amino acids after induction of knee joint inflammation in rats using kaolin and carrageenan. During the initial 10-min collection after knee joint injection with the irritants, the concentration of glutamate and the nitric oxide metabolites, arginine and citrulline, doubled. This increase persisted for at least two hours. During the same time period aspartate concentrations remained unchanged. Direct knee joint administration of lidocaine prevented the increases in amino acid concentration measurable by microdialysis probe inserted into the joint. These data suggest the possibility that glutamate may be released by neuronal endings in the joint.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acids / analysis
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Amino Acids / metabolism*
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Anesthetics, Local / administration & dosage
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Anesthetics, Local / pharmacology
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Animals
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Arginine / metabolism
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Arthritis / chemically induced
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Arthritis / metabolism*
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Carrageenan
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Chromatography, High Pressure Liquid
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Citrulline / metabolism
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Glutamic Acid / metabolism
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Hindlimb / physiology
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Injections, Intra-Articular
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Joints / chemistry
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Joints / metabolism*
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Kaolin
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Lidocaine / administration & dosage
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Lidocaine / pharmacology
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Male
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Pain / metabolism*
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Rats
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Rats, Sprague-Dawley
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Synovial Fluid / drug effects
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Synovial Fluid / metabolism
Substances
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Amino Acids
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Anesthetics, Local
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Kaolin
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Citrulline
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Glutamic Acid
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Carrageenan
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Arginine
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Lidocaine