Abstract
Autophagic and proteasomal proteolysis are two major pathways for degradation of cellular constituents. Current models suggest that autophagy is responsible for the nonselective bulk degradation of long-lived proteins and organelles while the proteasome specifically degrades short-lived proteins including misfolded proteins caused by the absence of Hsp90 function. Here, we show that the IκB kinase (IKK), an essential activator of NF-κB, is selectively degraded by autophagy when Hsp90 is inhibited by geldanamycin (GA), a specific Hsp90 inhibitor showing highly effective anti-tumor activity. We find that in this case inactivation of ubiquitination or proteasome fails to block IKK degradation. However, inhibition of autophagy by an autophagy inhibitor or knockout of Atg5, a key component of the autophagy pathway, significantly rescues IKK from GA-induced degradation. These findings provide the first evidence that an Hsp90 client may be degraded by a mechanism different from the proteasome pathway and establish a molecular link among Hsp90, NF-κB and autophagy
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Introduction
Cellular homeostasis requires a well-controlled balance between protein synthesis and degradation. While protein degradation is mediated primarily by the ubiquitin-proteasome and autophagy-lysosome pathways, protein synthesis involves a series of processes, including mRNA transcription, protein translation, protein folding/maturation and subsequent conformation maintenance 1, 2. The correct folding and stability of a number of signaling molecules, including many kinases and transcription factors, requires the molecular chaperone Hsp90 (heat-shock protein of 90 kDa) and its co-chaperones 3, 4. Specific inhibition of Hsp90 chaperone function by geldanamycin (GA), an anti-tumor drug, leads to degradation of its clients 4, 5. So far, the degradation of all known Hsp90 clients induced by GA is mediated by the proteasome 3, 4. Yet, to date, it has not been established whether the autophagy-lysosome pathway plays a role in GA-induced degradation of Hsp90 clients.
In mammals, three modes of autophagy have been identified: macroautophagy, microautophagy and chaperone-mediated autophagy (CMA). These three modes differ with respect to the pathway by which cytoplasmic material is delivered to the lysosome, but share in common the final steps of lysosomal degradation of the cargo with eventual recycling of the degraded material 1, 6. In CMA, the substrate protein is specifically recognized by a chaperone complex containing Hsc70 (heat-shock cognate of 70 kDa) and then delivered into the lysosome. In microautophagy, the cargo is engulfed directly at the lysosomal surface by invagination, protusion and/or septation of the lysosomal membrane. In contrast, macroautophagy is characterized by the fact that the cargo is sequestered into a double membrane structure termed autophagosome before delivery to the lysosome. Macroautophagy is the main form of autophagy and usually referred to directly as autophagy (same herein).
Recently, the IκB kinase (IKK) has been found to be a novel client of Hsp90 7. IKK is a protein complex composed of three subunits, IKKα (IKK1), IKKβ (IKK2) and IKKγ (NEMO), which directly phosphorylates IκBs (inhibitors of NF-κB) for subsequent proteasomal degradation. The degradation of IκBs leads to activation of NF-κB, a transcription factor family involved in diverse biological processes 8, 9. Here, we demonstrate that all three subunits of IKK are selectively degraded by autophagy when Hsp90 is inhibited by GA. We find that inactivation of ubiquitination or proteasome fails to block IKK degradation induced by GA. However, biochemical or genetic inhibition of the autophagic pathway significantly rescues IKK from GA-induced degradation.
Materials and Methods
Expression vectors and reagents
Expression vectors encoding IKK have been described as before 10. The anti-Hsp90 (F-8) and anti-p53 (FL-393) antibodies were from Santa Cruz Biotech Inc. The anti-actin antibody (AAN01) was from Cytoskeleton Inc. Other antibodies were described previously 11, 12, 13, 14. MG132 and 5-aminoimidazole-4-carboxamide 1-b-D-ribofuranoside (AICAR) were from Calbiochem and Biomol, respectively.
Cell culture and transfection
Human B-cell line Ramos RG69, mouse fibroblasts ts20 and Atg5 knockout mouse embryonic fibroblasts (MEFs) were gifts from Drs Covey L, Ozer HL and Mizushima N, respectively. Human kidney 293 cells and Jurkat cells were described previously 15. 293, ts20 and MEF cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 2 mM L-glutamine. Ramos RG69 cells and Jurkat cells were cultured in RPMI supplemented with 10% fetal bovine serum and 2 mM L-glutamine. ts20 cells were usually maintained at 35 °C instead of 37 °C. For inactivation of E1 in the ts20 cells, the culture temperature was shifted to 39 °C. 293, ts20 and MEF cells were transfected with DEAE-Dextran and LipofectAMINE 2000 (Invitrogen), respectively 16, 17.
Immunoblotting
Cells were lysed in radioimmuoprecipitation assay buffer (RIPA buffer) (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.25% Na-deoxycholate, 1% NP-40, 1 mM dithiothreitol and 1 mM phenylmethylsulfony fluoride). About 30 μg whole-cell lysates were fractionated by SDS-PAGE, transferred to nitrocellulose membranes and subjected to immunoblotting (IB) using the indicated antibodies 11, 15. To detect MG132 recovery of Akt from GA-mediated degradation by IB assay, the whole-cell lysates were prepared with the RIPA buffer containing 1% SDS 18.
Polysome and RNA isolation
Ramos RG69 cells were treated for 2 h with anti-CD40 antibody (10 μg/ml) or GA (2 μM) or left untreated. 20% of treated or untreated cells (2 × 107 cells/each group) were pelleted and employed as a source for total RNA using Trizol reagent (Invitrogen). The remaining cells were incubated with cycloheximide (100 μg/ml) for 15 min, followed by cytoplasm extraction. The cytoplasmic extract was then loaded onto a linear 10-45% (w/w) sucrose gradient and centrifuged for 2 h 30 min at 36 000 rpm. with a Beckman SW-41 rotor. After centrifugation, the gradient was fractionated and its absorbance at 254 nm was determined continuously by an Isco UA-5 monitor as described before 13. The polysome-containing fractions were pooled and subjected to phenol extraction. RNA was precipitated with ethanol and dissolved in DEPC-treated water.
Real-time reverse transcription-PCR analysis
Two micrograms of total RNA or RNA isolated from polysomes were reverse-transcribed for real-time reverse transcription (RT)-PCR using the following primers:
human IKKα: forward 5′-CCA CTA TGC TGA GGT TGG TGT, reverse 5′-AGT CTC CCT GAC GTC TTC CAT;
human IKKβ: forward 5′-TAG CAT GAA TGC CTC TCG ACT, reverse 5′-TTC AGC CAC CAG TTC TTC ACT;
human IKKγ: forward 5′-TAT CTA CAA GGC GGA CTT CCA, reverse 5′-TGG CCT TCA GTT TGC TGT ACT;
human p100: forward 5′-TGC CAT TGT GTT CCG GAC A, reverse 5′-TGT TTG GAA TCA GAC ACG TCC C;
human GAPDH: forward 5′-GCA AAT TCC ATG GCA CCG T, reverse 5′-TCG CCC CAC TTG ATT TTG G.
Real-time PCR assays were performed with an ABI Prism 7900HT sequence detection system using the SYBR Green PCR Core Reagent (Applied Biosystems, Foster City, CA) 13.
Results
Hsp90 is required for protein expression of IKK
Although it is clear that Hsp90 physically associates with IKK, the role of Hsp90 in IKK expression is still controversial 7, 19. To address this discrepancy, we re-examined the effect of GA treatment on IKK expression in numerous cell lines. As shown in Figure 1, GA induced a significant decline of the expression levels of all three IKK subunits in all the cell lines we examined, including 293 cells, HeLa cells, B cells and T cells (also see Figures 2 and 3). The effect of GA was specific, because it had no effect on the expression levels of actin as well as p65 and p100, two members of NF-κB (bottom panels, and data not shown). Thus, it seems that Hsp90 plays a general role in IKK protein expression.
Hsp90 is not required for transcription or translation of IKK
To explore the mechanism of the GA-mediated decrease of IKK protein levels, we first examined whether GA suppressed IKK mRNA transcription and translation by performing the real-time RT-PCR and polysome fractionation analysis. As a control, the transcription and translation of p100 mRNA was also included. In agreement with the fact that CD40L can activate NF-κB to induce p100 expression, we found that CD40 antibody treatment dramatically enhanced p100 mRNA transcription and subsequent protein translation (Figure 4, columns 6). On the other hand, GA treatment hardly influenced mRNA transcription and translation of all three IKK subunits (columns 2-4). These data suggested that the decrease of IKK levels is due to GA-induced protein degradation of IKK.
IKK degradation induced by GA is independent of the ubiquitination and proteasome
Since all known Hsp90 clients are degraded by the ubiquitin-proteasome pathway when Hsp90 function is inhibited 4, we investigated the possible role of ubiqutination in GA-mediated degradation of IKK by using ts20 cells. ts20 cells are temperature-sensitive (ts) mutant cells expressing a heat-labile ubiquitin-activating enzyme (E1), therefore, defective in the ubiquitin pathway upon heat shock 20. Surprisingly, heat shock failed to prevent GA-mediated degradation of IKKα, IKKβ or IKKγ in these mutant cells (Figure 2A, lane 4). This unexpected result was not due to inefficient inactivation of E1, because heat shock completely blocked the degradation of p53 and IκBα (lane 6). These results demonstrated that the ubiquitin system is not required for GA-induced IKK degradation.
These results led us to further examine the role of the proteasome in the GA-induced IKK degradation, although the proteasome can mediate either ubiquitin-dependent or -independent proteolysis 21. To achieve this goal, we inhibited the proteolytic activity of the proteasome using MG132, a specific inhibitor of the proteasome. Consistent with the essential role of the proteasome in the degradation of IκBα, the direct target of IKK and major inhibitor of NF-κB 8, addition of MG132 dramatically increased expression level of IκBα (Figure 2B, lanes 3 and 5). However, the addition of MG132 failed to increase expression levels of IKKs (lane 5). Most importantly, MG132 could not block GA-induced IKK degradation (lane 3). It is noteworthy that GA treatment alone also significantly enhanced IκBα expression (lane 2), which was correlated perfectly with the IKK degradation induced by GA. Collectively, these studies clearly demonstrated that IKK degradation in the absence of Hsp90 function is independent of both ubiquitination and proteasome, further suggesting that an unidentified mechanism is responsible for the degradation of this Hsp90 client.
GA-mediated IKK degradation is largely mediated by autophagy
To define the novel mechanism by which IKK is degraded in the absence of Hsp90 function, we examined the possible role of autophagy, because it is the other main system responsible for protein degradation in addition to the proteasome 22, 23, 24. As shown in Figure 3A, AICAR, an inhibitor of autophagy 25, efficiently prevented IKK degradation induced by GA (top three panels, lane 3). The role of AICAR is specific, because it failed to protect Akt, a well-known client of Hsp90, from GA-mediated degradation (middle panel, lane 3). Consistent with previous studies showing that GA-induced degradation of Akt depends on the proteasome 18, inhibition of the proteasome by MG132 rescued Akt from the proteasomal proteolysis (middle panel, lane 4). These studies suggested that autophagy may be responsible for the GA-mediated degradation of IKK.
To validate these biochemical studies, we utilized the Atg5 deficient cells. Atg5 is essential for autophagosome formation, and knockout of Atg5 blocks autophagy 26. Consistent with the results shown above, knockout of Atg5 also significantly inhibited GA-triggered degradation of IKK (Figure 3B, top three panels, lane 4). In sharp contrast, Atg5 was not required for GA-induced Akt degradation, because Akt was still degraded in the Atg5 null cells (middle panel, lane 4). Thus, IKK degradation in the absence of Hsp90 is largely mediated by autophagy. This is the first evidence that autophagy may function as an alternative mechanism for Hsp90 client degradation when Hsp90 function is absent.
Discussion
The proteasome and autophagy are two highly conserved mechanisms that are primarily employed for protein degradation within eukaryotes. It is generally believed that autophagy is in principle a nonselective, bulk degradation system of long-lived proteins and organelles; while the proteasome specifically degrades short-lived proteins, including regulatory proteins and misfolded proteins caused by the absence of Hsp90 function. Here, we show that IKK is selectively degraded by autophagy but not by the proteasome when Hsp90 function is inhibited. This study thus provides the first line of evidence showing that autophagy may also selectively degrade proteins and an Hsp90 client can be targeted for degradation via a mechanism distinct from the proteasome.
Consistent with our findings, a recent study clearly demonstrates that catalase is selectively degraded by autophagy upon caspase inhibition. Interestingly, activation of autophagy by nutrient deprival, a prototypic stimulus of autophagy, fails to trigger catalase degradation 27. Together the emerging evidence strongly indicates that autophagic degradation can be highly selective and regulated, at least under certain situations. Right now, the mechanism by which autophagy specifically selects its cargo is still unknown. One can speculate that the substrate must either be modified before being recognized, or it may contain a specific sequence that can be directly recognized by the autophagy machinery. However, such modification or motif has not yet been identified. Similarly, it is unknown which autophagy gene products are involved in targeting specific proteins. The other possibility is that certain protein or protein complex is responsible for the selection. For example, the chaperone protein complex containing Hsp90 and Hsc70 has been found to recognize and deliver specific proteins directly to lysosome for degradation, a process called CMA 28. However, it is obvious that the IKK degradation induced by GA is not mediated by CMA, because the GA treatment actually disrupts the association between IKK and Hsp90 7, 19. More importantly, the IKK degradation requires Atg5 (Figure 3), a key player in autophagosome formation 26. On the other hand, CMA does not require ATG5/autophagosome and vesicular traffic 6. Given the significance of autophagy-mediated IKK degradation, it will be of great interest to determine the molecular basis of selective action of autophagy.
Autophagy and Hsp90 both contribute to cell survival under stress conditions. Paradoxically, they function oppositely at the molecular level 3, 4, 22, 23, 24, 29, 30. For example, autophagy degrades protein, while Hsp90 is required for stabilization of many proteins by assisting the folding and maintenance of the newly translated proteins. Additionally, autophagy suppresses tumor development, whereas Hsp90 facilitates tumor progression and renders tumor cells resistant to a variety of apoptosis-inducing stimuli such as radiation. The data in the present study establish a molecular link between autophagy and Hsp90 and demonstrate that IKK/NF-κB serves as the nexus in between. In this novel signaling pathway, Hsp90 positively regulates IKK stability and NF-κB activation. When Hsp90 function is lost (such as inhibition by GA), IKK proteins cannot be folded correctly and are accordingly degraded by autophagy, resulting in NF-κB inactivation (Figure 5). Given a causative role of NF-κB in tumorigenesis and resistance of malignant cells to apoptosis-based tumor surveillance 8, 9, our data here thus may provide an important insight into the molecular mechanism of the tumor suppression function of autophagy and the anti-tumor activity of GA. As GA has been used in clinic trials for metastatic cancers, the rationale may also be applicable to all the NF-κB associated diseases such as various tumors and autoimmune diseases.
Abbreviations
- AICAR:
-
5-aminoimidazole-4-carboxamide 1-b-D-ibofuranoside
- CMA:
-
chaperone-mediated autophagy
- GA:
-
geldanamycin
- Hsc70:
-
heat-shock cognate of 70 kDa
- Hsp90:
-
heat-shock protein of 90 kDa
- IκB:
-
inhibitor of NF-κB
- IKK:
-
IκB kinase
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Acknowledgements
We thank Drs Covey L for the Ramos cells, Mizushima N for Atg5 null MEFs and Ozer HL for ts20 cells. This study was assisted partially by research grants from the New Jersey Commission on Cancer Research, Fifth District AHEPA Cancer Research Foundation, American Cancer Society RSG MGO-110116 and National Institutions of Health 1R01 CA116616 to GX.
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Qing, G., Yan, P. & Xiao, G. Hsp90 inhibition results in autophagy-mediated proteasome-independent degradation of IκB kinase (IKK). Cell Res 16, 895–901 (2006). https://doi.org/10.1038/sj.cr.7310109
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DOI: https://doi.org/10.1038/sj.cr.7310109
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