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Conditioning Regimens

Conditioning regimens for the treatment of experimental arthritis with autologous bone marrow transplantation

Bone Marrow Transplantation volume 25, pages 357–364 (2000)Cite this article

Abstract

Adjuvant induced arthritis (AA) in Buffalo rats is a chronic progressive disease that responds very well to treatment with myeloablation and rescue with autologous BM. These previous results were obtained by conditioning with a lethal single dose of TBI. In the present study various other conditioning regimens were compared with single dose TBI. Fractionated TBI using adjusted total dose was equally effective. CY and BU when used as single agents at the highest tolerated dose were less effective. Combinations of CY (2 × 60 mg/kg) with lower dose (4 Gy) TBI and of BU with CY were as beneficial as high-dose TBI. These results indicate that a very intense reduction of T lymphocytes, in the order of 3–4 log, is required for obtaining the highest rate of long-lasting complete remissions. A similar conclusion was reached from our studies of various conditioning regimens in rats suffering from experimental allergic encephalitis (EAE). If extrapolated to the clinic, such a degree of T lymphocyte eradication poses upper limits to the number of T lymphocytes that can be safely reintroduced with the autograft. The exact limits cannot be derived from these experiments because the addition of autologous T lymphocytes to the graft yielded different results in the two models of autoimmune disease (AID). Bone Marrow Transplantation (2000) 25, 357–364.

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Acknowledgements

The author gratefully acknowledges the skilled technical assistance of Els Kinwel-Bohré. The rats used in this study were paid for by the Dutch Rheuma Fund.

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  1. Introgene BV, Leiden, The Netherlands

    DW van Bekkum

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van Bekkum, D. Conditioning regimens for the treatment of experimental arthritis with autologous bone marrow transplantation. Bone Marrow Transplant 25, 357–364 (2000). https://doi.org/10.1038/sj.bmt.1702153

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