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Collagen antibody-induced arthritis

Nature Protocols volume 1, pages 2512–2516 (2006)Cite this article

Abstract

Collagen antibody-induced arthritis (CAIA) is a simple mouse model of rheumatoid arthritis that can be used to address questions of pathogenic mechanisms and to screen candidate therapeutic agents. Arthritis is stimulated by the administration of a cocktail of monoclonal antibodies that are directed to conserved auto-antigenic epitopes in collagen type II, followed by endotoxin. The antibody-induced arthritis model offers several key advantages over the classic collagen-induced arthritis (CIA) model. These include rapid disease onset, high uptake rate, synchronicity, and the capacity to use genetically modified mice, such as transgenics and knockouts. This protocol takes 1–2 weeks to be completed.

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Figure 1: Schematic representation of type II collagen protein structure and the four monoclonal antibody epitopes in region CB11.
Figure 2: Dz13 inhibits synovial inflammatory cell infiltration in arthritic mice.

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Authors and Affiliations

  1. Department of Pathology, Centre for Vascular Research, School of Medical Sciences, The University of New South Wales, Sydney, 2052, NSW, Australia

    Levon M Khachigian

  2. Department of Haematology, The Prince of Wales Hospital, 2052, Sydney, Australia

    Levon M Khachigian

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  1. Levon M Khachigian
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Correspondence to Levon M Khachigian.

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Khachigian, L. Collagen antibody-induced arthritis. Nat Protoc 1, 2512–2516 (2006). https://doi.org/10.1038/nprot.2006.393

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