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Serum amyloid P component controls chromatin degradation and prevents antinuclear autoimmunity

Nature Medicine volume 5, pages 694–697 (1999)Cite this article

Abstract

Serum amyloid P component (SAP), a highly conserved plasma protein named for its universal presence in amyloid deposits1, is the single normal circulating protein that shows specific calcium-dependent binding to DNA and chromatin in physiological conditions2,3. The avid binding of SAP displaces H1-type histones and thereby solubilizes native long chromatin, which is otherwise profoundly insoluble at the physiological ionic strength of extracellular fluids4. Furthermore, SAP binds in vivo both to apoptotic cells5, the surface blebs of which bear chromatin fragments6, and to nuclear debris released by necrosis7. SAP may therefore participate in handling of chromatin exposed by cell death2,3,4,7. Here we show that mice with targeted deletion of the SAP gene8 spontaneously develop antinuclear autoimmunity and severe glomerulonephritis, a phenotype resembling human systemic lupus erythematosus, a serious autoimmune disease. The SAP–/– mice also have enhanced anti-DNA responses to immunization with extrinsic chromatin, and we demonstrate that degradation of long chromatin is retarded in the presence of SAP both in vitro and in vivo. These findings indicate that SAP has an important physiological role, inhibiting the formation of pathogenic autoantibodies against chromatin and DNA, probably by binding to chromatin and regulating its degradation.

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Figure 1: Glomerulonephritis in SAP-deficient mice.
Figure 2: Accelerated chromatin degradation in SAP-deficient mice, shown by whole-body clearance of radioactivity after injection of radio-iodinated long chromatin.

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Acknowledgements

This work was supported by UK Medical Research Council Programme Grant G79700510 to M.B.P. and by The Arthritis & Rheumatism Council.

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Authors and Affiliations

  1. Immunological Medicine Unit, Imperial College School of Medicine, Hammersmith Hospital, London, W12 0NN, UK

    M.C.M. Bickerstaff, W.L. Hutchinson, J. Herbert, G.A. Tennent, A. Bybee & M.B. Pepys

  2. Rheumatology Section, Imperial College School of Medicine, Hammersmith Hospital, London, W12 0NN, UK

    M.C.M. Bickerstaff, M. Botto, D.A. Mitchell & M.J. Walport

  3. Department of Histopathology Imperial College School of Medicine, Hammersmith Hospital, London, W12 0NN, UK

    H.T. Cook

  4. MRC Laboratory of Molecular Biology, Cambridge , CB2 2QH, UK

    P.J.G. Butler

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Bickerstaff, M., Botto, M., Hutchinson, W. et al. Serum amyloid P component controls chromatin degradation and prevents antinuclear autoimmunity. Nat Med 5, 694–697 (1999). https://doi.org/10.1038/9544

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