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Defective processing and presentation of exogenous antigens in mutants with normal HLA class II genes

Nature volume 343, pages 71–74 (1990)Cite this article

Abstract

PRESENTATION of an exogenous protein antigen to helper (CD4+) T-lymphocytes by antigen presenting cells (APC) generally requires that the APCs degrade the native protein antigen into an immunogenic peptide, a process termed 'antigen processing', and that this peptide bind to a major histocompatibility complex (MHC) class II molecule1–3. The complex of peptide and MHC molecule on the APC surface provides the stimulatory ligand for the αβJ T cell receptor4. The intracellular pathways and molecular mechanisms involved in the generation of the peptide-MHC complex are not well understood. Here, we describe several mutant APCs which are altered in their ability to present native exogenous protein antigens but effectively present immunogenic peptides derived from these proteins. The lesions in these mutants are not in the class II structural genes, but they affect the conformation of mature class II dimers.

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Authors and Affiliations

  1. Department of Pediatrics, University of Washington, Seattle, Washington, 98195, USA

    Elizabeth Mellins, Laura Smith, Benjamin Arp, Tom Cotner & Donald Pious

  2. Department of Geneticst, University of Washington, Seattle, Washington, 98195, USA

    Donald Pious

  3. Department of lmmunology, University of Washington, Seattle, Washington, 98195, USA

    Donald Pious

  4. T-Cell Sciences, Cambridge, Massachusetts, 02139, USA

    Esteban Celis

Authors
  1. Elizabeth Mellins
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  2. Laura Smith
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  3. Benjamin Arp
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  4. Tom Cotner
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  5. Esteban Celis
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  6. Donald Pious
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Mellins, E., Smith, L., Arp, B. et al. Defective processing and presentation of exogenous antigens in mutants with normal HLA class II genes. Nature 343, 71–74 (1990). https://doi.org/10.1038/343071a0

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