Original articleCardiovascularParacrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction
Section snippets
Material and Methods
All experiments were performed in accordance with the “Principles of Laboratory Animal Care” formulated by the National Society for Medical Research and the “Guide for the Care and Use of Laboratory Animals” prepared by the Institute of Laboratory Animal Resources, Commission on Life Science, National Research Council, and published by the National Academy Press, revised 1996.
Identification of Transplanted Mesenchymal Stem Cells
To identify grafted MSCs in the ischemic myocardium, MSCs were prelabeled with BrdU in the nucleus. 5-Bromo-2-deoxyruidine staining of MSCs demonstrated that 85% of MSCs were labeled with BrdU (Fig 1B). Transplanted cells were localized within the ischemic myocardium in MSC-transplanted hearts by means of immunohistochemical staining with monoclonal antibodies against BrdU (Fig 1C).
Capillary Density
Capillary density was quantified in the border zone of infarct area and compared between two groups. Mesenchymal
Comment
In this investigation, our data show that neovascularization induced in the ischemic myocardium is directly related to the paracrine action of engrafted MSCs in ischemic myocardium. The release of bFGF, VEGF, and SDF-1α leads to efficient vascular regeneration and also attenuates the apoptotic pathway. These paracrine actions account for reduced remodeling and recovery of cardiac performance.
We demonstrated that graft MSCs in ischemic myocardium secrete SDF-1α. This cytokine may have an effect
Acknowledgments
Yao Liang Tang is supported by an American Heart Association postdoctoral fellowship (0325378B). This work was supported by the National Institutes of Health MERIT Award HL 27339 (MIP). We are grateful for the expert technical assistance of Guoqian Huang, MD, Chuizheng Pan, and Xianhong Shu, MD.
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