A simplified measurement of degraded collagen in tissues: Application in healthy, fibrillated and osteoarthritic cartilage

doi:10.1016/S0945-053X(97)90012-3

Matrix Biology

Volume 16, Issue 5, November 1997, Pages 233-243

https://doi.org/10.1016/S0945-053X(97)90012-3 Get rights and content

Abstract

Intact triple helical collagen molecules are highly resistant to proteolytic enzymes, whereas degraded (unwound) collagen is easily digested. This fact was exploited to develop a simplified method for the quantification of the amount of degraded collagen in the collagen network of connective tissues. Essentially, the method involves extraction of proteoglycans with 4 M guanidinium chloride, selective digestion of degraded collagen by α-chymotrypsin, hydrolysis in 6 M HCl of the released fragments as well as the residual tissue, and then measurement of the amount of hydroxyproline in both pools. Since the digestion of degraded collagen by α-chymotrypsin and measurement of hydroxyproline is not restricted to a specific collagen type, this technique can be applied to a wide variety of connective tissues. The method was validated with articular cartilage.

Levels of in situ degraded collagen were about four-fold higher in degenerated (fibrillated) cartilage than in its healthy counterpart derived from the same donor. More detailed investigations revealed that the collagen damage in degenerated cartilage is more extensive at the cartilage surface than in the region adjacent to bone. This was not the case in healthy cartilage; identical low values were obtained at the surface and close to the bone. An impaired collagen network has been hypothesized to be the reason for the swelling of cartilage in osteoarthritis (OA). The present paper presents the first experimental evidence to support this hypothesis: more damage to the collagen network (i.e., more degraded collagen molecules within fibrils) is linearly related to more extensive swelling of the OA tissue in hypotonic saline.

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Regular papersA simplified measurement of degraded collagen in tissues: Application in healthy, fibrillated and osteoarthritic cartilage

Abstract

Matrix

J. Chromatogr.

Sem. Arthr. Rheum.

Osteoarthr. Cartil.

Anal. Biochem.

Biochem. Biophys. Res. Commun.

Anal. Biochem.

Matrix

Biochim. Biophys. Acta

Arch. Biochem. Biophys.

Clin. Chim. Acta

Anal. Biochem.

The human lumbar intervertebral disc

Changes in cartilage composition and physical properties due to stromelysin degradation

Arthr. Rheum.

Regular papers
A simplified measurement of degraded collagen in tissues: Application in healthy, fibrillated and osteoarthritic cartilage