Elsevier

Gene

Volume 152, Issue 2, 23 January 1995, Pages 221-226
Gene

Structural organization, expression and chromosomal mapping of the mouse cystatin-C-encoding gene (Cst3)

https://doi.org/10.1016/0378-1119(94)00728-BGet rights and content

Abstract

Cystatin C (CstC) is a potent cysteine-proteinase inhibitor. The structure of the mouse CstC-encoding gene (Cst3) was examined by sequencing a 6.1-kb genomic DNA containing the entire gene, as well as 0.9 kb of 5′ flanking and 1.7 kb of its 3′ flanking region. The sequence revealed that the overall organization of the gene is very similar to those of the genes encoding human CstC and other type-2 Cst, with two introns at positions identical to those in the human gene. The promoter area does not contain typical TATA or CAAT ☐es. Two copies of a Spl-binding motif, GGGCGG, are present in the 5′ flanking region within 300 bp upstream from the initiation codon. A hexa-nucleotide, TGTTCT, which is a core sequence of the androgen-responsive element (ARE), is found in the promoter region. This region also contains a 21-nucleotide sequence, 5′-AGACTAGCAGCTGACTGAAGC, which contains two potential binding sites for the transcription factor, AP-1. The mouse Cst3 mRNA was detected in all of thirteen tissues examined by Northern blot analysis. Cst3 was mapped in the mouse to a position on distal chromosome 2.

Keywords

Recombinant genomic DNA
gene mapping
exon
intron
promoter sequence
proteinase inhibitor
multigene family
aa
amino acid(s)
ARE
androgen-responsive element(s)
bp
base pair(s)
Chr
chromosome
Cst
cystatin(s)
Cst3
gene (DNA) encoding CstC
Cstl
gene (DNA) encoding CstSN
Cst2
gene (DNA) encoding CstSA
Cst4
gene (DNA) encoding CstD
HCCAA
hereditary CstC amyloid angiopathy
kb
kilobase(s) or 1000 bp
nt
nucleotide(s)
RFLP
restriction-fragment length polymorphism
W
A or T

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