Flow-cytometric determination of peptide-class I complex formation identification of p53 peptides that bind to HLA-A2
References (35)
- et al.
Putting together an MHC class I molecule
Curr Opin Immunol
(1993) - et al.
Antigen presentation: structural themes and functional variations
Immunol Today
(1991) - et al.
Eradication of adenovirus E1A-induced tumors by E1A-specific cytotoxic T lymphocytes
Cell
(1989) - et al.
Structure of the gene encoding for tum− transplantation antigen P91A: a peptide encoded by the mutated exon is recognized with Ld by cytolytic T cells
Cell
(1989) Toward a genetic analysis of tumor rejection antigens
Adv Cancer Res
(1992)- et al.
A method to quantify binding of unlabeled peptides to class I MHC molecules and detect their allele specificity
J Immunol Methods
(1993) - et al.
An HLA-A2/beta-2-microglobulin-peptide complex assembled from subunits expressed separately in Escherichia coli
Mol Immunol
(1992) - et al.
Binding of radioiodinated influenza virus peptides to class I MHC molecules and to other cellular proteins as analyzed by gel filtration and photoaffinity labeling
Mol Immunol
(1991) - et al.
p53 function and dysfunction
Cell
(1992) - et al.
Antigen recognition by class I restricted T lymphocytes
Annu Rev Immunol
(1989)
Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules
Nature
Identification of self peptides bound to purified HLA-B27
Nature
Isolation and analysis of naturally processed viral peptides as recognized by cytotoxic T cells
Nature
Lessons from T cell responses to virus induced tumors for cancer eradication in general
Cancer Surv
Recognition of human melanoma cells by HLA-A2. 1-restricted cytotoxic T lymphocytes is mediated by at least six shared peptide epitopes
J Immunol
Clonal deletion versus clonal anergy: the role of the thymus in inducing self tolerance
Science
Limit of T cell tolerance to self peptides by peptide presentation
Science
Cited by (75)
Occupational health effect of TCE exposure: Experiment evidence of gene-environment interaction in hypersensitivity reaction
2022, Chemico-Biological InteractionsA synthetic DNA template for fast manufacturing of versatile single epitope mRNA
2022, Molecular Therapy Nucleic AcidsCitation Excerpt :Therefore, this sorting signal was fused to the cDNA encoding a 27-mer peptide, consisting of the immunodominant 9-mer epitope under evaluation, which is flanked at the 5′ end and 3′ end by additional protein 9-mers. We evaluated presentation of the HLA-DP4-restricted epitope of the shared antigen MAGE-A3 (TQHFVQENYLEY)34,35 and the HLA-A2-restricted epitopes of the shared antigens gp100 (YLEPGPVTA),36–39 NY-ESO-1 (SLLMWITQC),40–42 and p53 (LLGRNSFEV).43,44 We electroporated HLA-A2+ moDCs with SDT-mRNA or PDT-mRNA and co-cultured them at a 1:1 ratio with CD8+ T cells that were electroporated with mRNA encoding the corresponding TCRα and TCRβ chain to test antigen presentation to CD8+ T cells (Table S3).
Identification of Autoantigen Epitopes in Alopecia Areata
2016, Journal of Investigative DermatologyCitation Excerpt :T2 cells are TAP-1 and -2 (transporter associated with antigen processing) deficient and only express stable HLA-A2 in the presence of high-affinity peptides; therefore, HLA-A2 stability correlates to peptide biological activity and affinity in vitro. T2 stabilization assays were performed following protocols described elsewhere (Forero et al., 1998; Zeh et al., 1994) with slight modifications (Supplementary Materials online). HLA-A2 restrictive Influenza M1 58-66 peptide (GILGFVFTL) was used as a positive control to indicate maximum HLA-A2 expression levels.
A modified epitope identified for generation and monitoring of PSA-specific T cells in patients on early phases of PSA-based immunotherapeutic protocols
2009, VaccineCitation Excerpt :Human C1R-A2 cells (stably transfected with episomal vector pHebo-A2 to express HLA-A*0201) were kindly provided by Dr. R. Kiessling (Cancer Center Karolinska, Karolinska Institute, Stockholm, Sweden), and were cultured in RPMI 1640 medium supplemented with 10 mM HEPES, 2 mM l-glutamine, 25 μg/ml gentamycin, 10% FCS and 250 μg/ml selective antibiotic Hygromycin B (Roche Diagnostics GmbH, Mannheim, Germany). The HLA-A*0201 reconstitution assay using C1R-A2 cells was performed as described previously [19] with a few modifications. Briefly, C1R-A2 cells were washed in ice cold PBS and the cell pellet was cooled on ice for 5 min.
Generation of CA125-specific cytotoxic T lymphocytes in human leukocyte antigen-A2.1-positive healthy donors and patients with advanced ovarian cancer
2009, American Journal of Obstetrics and GynecologyCitation Excerpt :Peptides were dissolved in DMSO and frozen at -80°C until use. Peptide-induced stabilization of HLA-A2.1 molecules on T2 cells was performed as described previously.14 The positive control consisted of T2 cells loaded with the influenza virus matrix peptide, GILGFVFTL.