Elsevier

Human Immunology

Volume 19, Issue 4, August 1987, Pages 269-278
Human Immunology

HLA DQα and DQβ restriction fragment length polymorphisms associated with Felty's syndrome and DR4-positive rheumatoid arthritis

https://doi.org/10.1016/0198-8859(87)90044-9Get rights and content

Abstract

HLA DQα and DQβ cDNA probes were used to study TaqI generated restriction fragment length polymorphisms (RFLPs) in DR4-positive patients with Fetly's syndrome (FS), seropositive rheumatoid arthritis (RA), and in HLA-DR4 positive controls. The results of this analysis revealed two DQβ RFLP patterns (DQβ3a and DQβ3b) associated with DR4, of which DQβ3b was found at significantly higher frequency in patients with FS (73%) or with RA (52%) than in DR4 controls (29%). Hind III generated RFLPs provide evidence that DQβ3b is in strong linkage disequilibrium with the gene encoding the serologically recognized epitope TA10. Results obtained using a DQα chain probe revealed polymorphic difference between DQα chain genes associated with different DR types, thereby providing a possible explanation for the lack of association between RA and other DR haplotypes in linkage disequilibrium with TA10. We conclude that both DQα and DQβ genes may be important in determining HLA-linked susceptibility to serve forms of RA.

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