Immunohistochemistry and ultrastructure of myoepithelium and modified myoepithelium of the ducts of human major salivary glands: Histogenetic implications for salivary gland tumors

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Abstract

The organization of salivary gland ducts, especially the presence or absence of myoepithelial cells, is central to histogenetic approaches to the classification of salivary gland tumors. Striated and excretory ducts are reported to be devoid of myoepithelial cells but do contain basal cells. To investigate the nature of such basal cells, tissue sections of normal human salivary glands were examined by means of immunohistochemical, ultrastructural, and fluorescent microscopic techniques. With the use of a mouse monoclonal anticytokeratin antibody (312C8-1) that, in salivary glands, is specific for myoepithelial cells, these cells associated with acini and intercalated ducts were strongly stained, as were the basal cells of striated and excretory ducts in each case. Ultrastructurally, some basal cells of both striated and excretory ducts had narrow, elongated cellular processes or the main portion of the cell containing parallel arrays of microfilaments with linear densities and micropinocytotic vesicles, whereas in other basal cells tonofilament bundles predominated. A similar range of cytoplasmic features existed in myoepithelial cells associated with acinar and intercalated duct cells. In addition, some duct basal cells have a complement of actin filaments similar to classic myoepithelium of acini and intercalated ducts. Striated and excretory ducts of human salivary glands, therefore, contain fully differentiated and modified myoepithelial cells, both of which express a specific cytokeratin polypeptide that is absent from duct luminal and acinar cells. Differentiation patterns in the intralobular and interlobular ducts suggest that these regions of salivary gland parenchyma cannot be excluded as histogenetic sites for the induction of salivary gland tumors in which neoplastic myoepithelial cells have been shown to have a major role.

References (51)

  • YoungJ.A. et al.

    The morphology of salivary glands

  • CutlerL.S. et al.

    Ultrastructure of the parotid duct. Cytochemical studies of the striated duct and papillary cystadenoma lymphomatosum of the human parotid gland

    Arch Pathol Lab Med

    (1977)
  • Testa-RivaF. et al.

    The epithelium of the excretory duct of the human submandibular gland: a transmission and scanning electron microscope study

    Am J Anat

    (1981)
  • GarrettJ.R. et al.

    Alkaline phosphatase and myoepithelial cells in the parotid gland of the rat

    Histochem J

    (1973)
  • TandlerB. et al.

    Ultrastructure of human labial salivary glands. III. Myoepithelium and ducts

    J Morphol

    (1970)
  • TandlerB.

    Unusual myoepithelium in the retrolingual salivary gland of the European hedgehog

    J Submicrosc Cytol

    (1986)
  • van LennepE.W. et al.

    The ultrastructure of the sheep parotid gland

    Cell Tissue Res

    (1977)
  • DairkeeS.H. et al.

    Monoclonal antibody that identifies human myoepithelium

  • PalmerR.W. et al.

    Immunocytochemical identification of cell types in pleomorphic adenoma, with particular reference to myoepithelial cells

    J Pathol

    (1985)
  • PalmerR.M. et al.

    “Epimyoepithelial” islands in lymphoepithelial lesions: an immunohistochemical study

    Virchows Arch (Pathol Anat)

    (1986)
  • PalmerR.M.

    The identification of myoepithelial cells in human salivary glands. A review and comparison of light microscopical methods

    J Oral Pathol

    (1986)
  • TakaiY. et al.

    Immunohistochemical detection of keratin proteins in salivary gland ducts of mammals

    Acta Histochem Cytochem

    (1985)
  • SchneyerC.A.

    β-Adrenergic effects of autonomic agents on mitosis and hypertrophy in rat parotid gland

  • EversoleL.R.

    Histogenic classification of salivary gland tumors

    Arch Pathol

    (1971)
  • EversoleL.R.

    Histogenisis of salivary gland neoplasms

    Otolaryngol Clin North Am

    (1977)
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    This study was funded by a grant from the Moe Levin Family Foundation, Montreal, Quebec, Canada. We appreciate the manuscript preparation by Marilyn McDougall and the technical assistance in fluorescent microscopy by Judy Little.

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