Research reportCytokine-based human whole blood assay for the detection of antigen-reactive T cells
References (36)
- et al.
Catalyzed reporter deposition, a novel method of signal amplification
J. Immunol. Methods
(1989) - et al.
Direct stimulation of cytokines (IL-1b, TNA-α, IFN-γ and GM-CSF) in whole blood. I. Comparison with isolated PBMC stimulation
Cytokine
(1992) A simplified microtechnique for measuring human lymphocyte proliferation after stimulation with mitogen and specific antigen
J. Immunol. Methods
(1988)- et al.
Inverse relationship between humoral and cellular immunity to glutamic acid decarboxylase in humans at-risk for insulin-dependent diabetes
Lancet
(1993) - et al.
Enhancing effects of autologous erythrocytes on human or mouse cytokine secretion and IL-2R expression
Cell. Immunol.
(1993) - et al.
A whole-blood technique for testing production of human interferon by leukocytes
J. Immunol. Methods
(1982) - et al.
Measurement of antigen-dependent interleukin-4 production by human peripheral blood mononuclear cells
J. Immunol. Methods
(1992) - et al.
A whole-blood lymphoproliferation assay for measuring cellular immunity against herpes viruses
J. Immunol. Methods
(1985) - et al.
Development of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity
J. Immunol. Methods
(1994) - et al.
A convenient human whole blood culture system for studying the regulation of tumour necrosis factor release by bacterial lipopolysaccharide
J. Immunol. Methods
(1991)
Correlation between in vitro cytokine production and clinical evolution of multiple sclerosis patients
Schweiz. Arch. Neurol. Psychiatr.
Natural regulators of T-cell lymphokine production in vivo
J. Immunother.
Secondary immunization with a protein antigen (tetanus toxoid) in man
Scand. J. Immunol.
Evaluation of a test system for measuring cytokine production in human whole blood cell cultures
J. Immunol. Methods
Impaired cytokine production in whole blood cell cultures of patients with gynaecological carcinomas in different clinical stages
Br. J. Cancer
Appraisal of the total blood lymphocyte proliferation assay as a diagnostic tool in screening for tuberculosis
J. Med. Microbiol.
Cytokine production in whole blood in vivo
Agents Actions
IL-10 inhibits cytokine production by activated macrophages
J. Immunol.
Cited by (57)
Evaluation of safety and immunogenicity of receptor-binding domain-based COVID-19 vaccine (Corbevax) to select the optimum formulation in open-label, multicentre, and randomised phase-1/2 and phase-2 clinical trials
2022, eBioMedicineCitation Excerpt :PSV neutralisation assay (PNA) and cellular immune responses analysis were conducted by measuring cytokine secretion using TrueCulture tubes (Q2 solutions, USA) coated with SARS-COV-2 peptides. Cellular immune responses were assessed in a subset of subjects using terms of cytokine secretion in TrueCulture tubes.18 Whole blood samples were incubated in TrueCulture tubes coated with SARS-COV-2 peptides (Myriad Inc., TX, USA).
Quantification of a cell-mediated immune response against varicella zoster virus by assessing responder CD4<sup>high</sup> memory cell proliferation in activated whole blood cultures
2019, VaccineCitation Excerpt :This is because whole blood assays have the advantage of being fast to perform, with more natural conditions that retain all blood components and maintain cells in their in vivo ratios. In the literature, previous whole blood-based assays showed varying stimulation periods that depended on the antigen, measured components, and culture conditions [18,24–28]. To determine the best time interval to measure the level of responder CD4-M proliferation, we performed a time-course analysis using blood from four donors with a history of varicella, but not HZ.
Overview of the Immune System and Immunotoxicology
2018, Comprehensive Toxicology: Third EditionWhole blood assay and visceral leishmaniasis: Challenges and promises
2014, ImmunobiologyCitation Excerpt :Protective immunity against Leishmania infection is predominantly T cell-mediated and results in the killing of intracellular parasites by activated macrophages and cytotoxic responses (Peruhype-Magalhaes et al. 2006). Several groups have developed WBA for measuring responses to mitogens (De Groote et al. 1992; Junge et al. 1970; Petrovsky and Harrison 1995), antigens (Paty and Hughes 1972; Pauly et al. 1973) and specific antigens for investigating a variety of infectious diseases in which T cell mediated immunity plays an important role including herpes virus (Leroux et al. 1985), various bacterial infections (Kaneene et al. 1978; Koskela and Herva 1980), leprosy (Weir et al. 1998, 1994, 1999) and tuberculosis (Diel et al. 2011; Fiavey and Frankenburg 1992). However, we have recently developed and optimized the whole blood interferon-γ release assay (IGRA) for VL, using soluble leishmania antigen (SLA); which permits the measurement of cytokines released from stimulated monocytes & T-cells (Ansari et al. 2012; Gidwani et al. 2011; Singh et al. 2012a).
Increased maternal cytokine production and congenital heart defects
2013, Journal of Reproductive Immunology