Crossed immuno-affinoelectrophoresis: An analytical method to predict the result of affinity chromatography

doi:10.1016/0003-2697(73)90215-7

Analytical Biochemistry

Volume 56, Issue 2, December 1973, Pages 480-488

https://doi.org/10.1016/0003-2697(73)90215-7 Get rights and content

Abstract

Crossed immuno-affinoelectrophoresis is described as an analytical technique in which the principles of electrophoresis and biospecific interaction are combined. By this method the results of affinity chromatographic experiments can be predicted. Human serum proteins were analyzed by crossed immuno-affinoelectrophoresis and affinity chromatography on concanavalin A bound to Sepharose and concordant results were obtained. Moreover crossed immuno-affinoelectrophoresis is suggested for identification of components with certain recognizable molecular structures.

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Citation Excerpt :
Purified human plasma α1-PI was used as a standard. The glycosylation pattern of α1-PI was analyzed by crossed affinoimmunoelectrophoresis (CAIE) with concanavalin A (ConA) according to the Bog-Hansen method [19], using 24.3 mM diethylphenobarbituric acid/Tris pH 8.6, containing 0.4 mM calcium lactate. At first, proteins were resolved by electrophoresis in agarose gel (3 h at 10 V/cm) incorporated with ConA (1.5 mg/ml).
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Crossed immuno-affinoelectrophoresis: An analytical method to predict the result of affinity chromatography

Abstract

Nature (London)

Z. Physiol. Chem.

J. Immunol. Methods

Bull. Yamaguchi Med. Sch.

Bull. Yamaguchi Med. Sch.

Comp. Biochem. Physiol.