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Antibodies to Citrullinated Vimentin are a Specific and Sensitive Marker for the Diagnosis of Rheumatoid Arthritis

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Abstract

Background

The last 5 years have seen the emergence and establishment of antibodies to citrullinated antigens as the diagnostic marker for rheumatoid arthritis (RA). Initially, these were detected using a synthetic peptide, which has undergone a number of modifications to give a diagnostic test with a sensitivity of 65–80% and a specificity of >95%. Antibodies to citrullinated vimentin were first described in 1994 as a highly specific marker for RA (anti-Sa). However, no easily performed assay for these antibodies has been available.

Methods

We have examined the use of a ELISA-based assay with a mutated citrullinated vimentin (MCV) antigen (Orgentec, Mainz, Germany) to assess the diagnostic and prognostic utility of this antibody in RA.

Results

Antibodies to MCV were detected in the sera of 74% RA patients (specificity 96%), 2% systemic lupus erythematosus, 14% Sjögren’s syndrome, and 2% scleroderma. Anti-MCV was not detected in sera from healthy blood donors. There was no difference in the frequency of antibodies detected in RA patients with early (<2 years) or chronic (>2 years) disease. There was no significant variation in anti-MCV antibody concentrations in early RA patients over a 52-week period. No significant change was observed with time between the two treatment groups of methotrexate alone or methotrexate plus infliximab.

Conclusions

Antibodies to MCV are a specific and sensitive marker for the diagnosis of RA.

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References

  1. Huizinga TWJ, Landewé RBM (2005) Nat Clin Pract Rheum 1(1):2–3

    Article  Google Scholar 

  2. Arnett FC, Edworthy SM, Bloch DA et al (1988) Arthritis Rheum 31:315–324

    Article  PubMed  CAS  Google Scholar 

  3. Cruyssen BV, Cantaert T, Nogueira L et al (2006) Arthritis Res Ther 8:R122

    Article  CAS  Google Scholar 

  4. Kinloch A, Tatzer V, Wait R et al (2005) Arthritis Res Ther 7:R1421–R1429

    Article  PubMed  CAS  Google Scholar 

  5. Vossenaar ER, Radstake TR, Van Der Heijden A et al (2004) Ann Rheum Dis 63:373–381

    Article  PubMed  CAS  Google Scholar 

  6. Tak PP, Smeets TJ, Daha MR et al (1997) Arthritis Rheum 40:217–225

    Article  PubMed  CAS  Google Scholar 

  7. Zendman AJW, van Venrooij WJ, Pruijn GJM (2006) Rheumatology 45:20–25

    Article  PubMed  CAS  Google Scholar 

  8. Tarcsa E, Marekov LN, Mei G et al (1996) J Biol Chem 271:30709–30716

    Article  PubMed  CAS  Google Scholar 

  9. Lundberg K, Nijenhuis S, Vossenaar E et al (2004) Arthritis Res Ther 6(Suppl 1):18 (Abstract)

    Article  Google Scholar 

  10. Hill JA, Southwood S, Sette A et al (2003) J Immunol 171:538–541

    PubMed  CAS  Google Scholar 

  11. van Gaalen F, Ioan-Facsinay A, Huizinga TWJ et al (2005) J Immunol 175:5575–5580

    PubMed  Google Scholar 

  12. Masson-Bessiere C, Sebbag M, Durieux JJ et al (2000) Clin Exp Immunol 119:544–552

    Article  PubMed  CAS  Google Scholar 

  13. Makrygiannakis D, af Klint E, Lundberg IE et al (2006) Ann Rheum Dis 65:1219–1222

    Article  PubMed  CAS  Google Scholar 

  14. Young BJ, Mallya RK, Leslie RD et al (1979) Br Med J 14:97–99

    Article  Google Scholar 

  15. Simon M, Girbal E, Sebbag M et al (1993) J Clin Invest 92:1387–1393

    PubMed  CAS  Google Scholar 

  16. Sebbag M, Simon M, Vincent C et al (1995) J Clin Invest 95:2672–2679

    PubMed  CAS  Google Scholar 

  17. Girbal-Neuhauser E, Durieux JJ, Arnaud M et al (1999) J Immunol 162:585–594

    PubMed  CAS  Google Scholar 

  18. van Venrooij WJ, Pruijn GJM (2000) Arthritis Res 2:249–251

    Article  PubMed  Google Scholar 

  19. Nielen MMJ, van der Horst AR, van Schaardenburg D et al (2005) Ann Rheum Dis 64:1199–1204

    Article  PubMed  CAS  Google Scholar 

  20. Schellekens GA, de Jong BA, van den Hoogen FH et al (1998) J Clin Invest 101:273–281

    Article  PubMed  CAS  Google Scholar 

  21. Quinn MA, Gough AK, Green MJ et al (2006) Rheumatology 45(4):478–480

    Article  PubMed  CAS  Google Scholar 

  22. van Gaalen FA, Visser H, Huizinga TWJ (2005) Ann Rheum Dis 64:1510–1512

    Article  PubMed  CAS  Google Scholar 

  23. Takasaki Y, Yamanaka K, Takasaki C et al (2004) Mod Rheumatol 14(5):367–375

    Article  PubMed  Google Scholar 

  24. Vannini A, Cheung K, Fusconi M et al (2006) Ann Rheum Dis (published online http://ard.bmj.com/cgi/content/abstract/ard.2006.058933v1)

  25. Rantapaa-Dahlqvist S, de Jong BA, Berglin E et al (2003) Arthritis Rheum 48(10):2701–2705

    Article  CAS  Google Scholar 

  26. Van Gaalen FA, Linn-Rasker SP, van Venrooij WJ et al (2004) Arthritis Rheum 50(3):709–715

    Article  PubMed  CAS  Google Scholar 

  27. Berglin E, Padyukov L, Sundin U et al (2004) Arthritis Res Ther 6:R303–R308

    Article  PubMed  CAS  Google Scholar 

  28. van der Helm-van Mil AHM, Verpoort KN, Breedveld FC et al (2005) Arthritis Res Ther 7(5):R949–R958

    Article  PubMed  CAS  Google Scholar 

  29. Berglin E, Johansson T, Sundin U et al (2006) Ann Rheum Dis 65(4):453–458

    Article  PubMed  CAS  Google Scholar 

  30. Inanc N, Dalkilic E, Kamali S et al (2007) Clin Rheumatol 26(1):17–23

    Article  PubMed  CAS  Google Scholar 

  31. Despres N, Boire G, Lopez-Longo FJ et al (1994) J Rheumatol 21(6):1027–1033

    PubMed  CAS  Google Scholar 

  32. Menard HA, Lapointe E, Rochdi MD et al (2000) Arthritis Res 2:429–432

    Article  PubMed  CAS  Google Scholar 

  33. Hueber W, Hassfeld W, Smolen JS et al (1999) Rheumatology 38:155–159

    Article  PubMed  CAS  Google Scholar 

  34. Hayem G, De Bandt M, Palazzo E et al (1999) J Rheumatol 26:7–13

    PubMed  CAS  Google Scholar 

  35. Vossenaar ER, Despres N, Lapointe E et al (2004) Arthritis Res Ther 6(2):R142–R150

    Article  PubMed  CAS  Google Scholar 

  36. Boire G, Cossette P, de Brum-Fernandes AJ et al (2005) Arthritis Res Ther 7:R592–R603

    Article  PubMed  CAS  Google Scholar 

  37. Bang H, Lûthke K, Gauliard A et al (2006) Ann Rheum DIs 65(Suppl II):144 (Abstract)

    Google Scholar 

  38. Lakos G, Soos L, Szabo Z et al (2006) Abstract THU0118, EULAR

  39. Klotz W, Dejaco C, Larcher H et al (2006) Abstract FRI0050, EULAR

  40. Brickmann KP, Yazdani-Biuki BJ, Graninger M et al (2006) Abstract SAT0431, EULAR

  41. Dejaco C, Klotz W, Larcher H et al (2006) Arthritis Res Ther 8:R119–R124

    Article  PubMed  CAS  Google Scholar 

  42. Hassfeld WG, Vinje O, König K et al (2006) Abstract FR10513, EULAR

  43. Brickmann K, Yazdani-Biuki BJ, Mûller T et al (2006) Abstract AB0492, EULAR

  44. Egerer K, Bang H, Gauliard A et al (2006) Poster presented at EULAR

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Authors and Affiliations

  1. Division of Immunology, Hammersmith Hospitals NHS Trust, London, UK

    Hannah Poulsom & Peter J. Charles

  2. Kennedy Institute of Rheumatology, Imperial College, London, UK

    Peter J. Charles

  3. Division of Immunology, Charing Cross Hospital, Fulham Palace Road, London, W6 8RF, UK

    Peter J. Charles

Authors
  1. Hannah Poulsom
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  2. Peter J. Charles
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Correspondence to Peter J. Charles.

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Poulsom, H., Charles, P.J. Antibodies to Citrullinated Vimentin are a Specific and Sensitive Marker for the Diagnosis of Rheumatoid Arthritis. Clinic Rev Allerg Immunol 34, 4–10 (2008). https://doi.org/10.1007/s12016-007-8016-3

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  • DOI: https://doi.org/10.1007/s12016-007-8016-3

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