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Expression of bone resorption genes in osteoarthritis and in osteoporosis

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Abstract

Cathepsin K and MMP-9 are considered to be the most abundant proteases in osteoclasts. TRAP is a marker for osteoclasts, and there is increasing evidence of its proteolytic role in bone resorption. RANKL is a recently discovered regulator of osteoclast maturation and activity and induces expression of many genes. This study compared cathepsin K, MMP-9, TRAP, RANKL, OPG, and osteocalcin gene expression in the proximal femur of patients with osteoarthritis with that of patients with femoral neck fracture. Fifty-six patients undergoing arthroplasty because of osteoarthritis or femoral neck fracture were included in the study. Total mRNA was extracted from the bone samples obtained from the intertrochanteric region of the proximal femur. Real-time RT-PCR was used to quantify CTSK (cathepsin K), MMP-9 (matrix metalloproteinase 9), ACP5 (TRAP), TNFSF11 (RANKL), TNFRSF11B (OPG), and BGLAP (osteocalcin) mRNAs. The levels of mRNAs coding for MMP-9 and osteocalcin indicated higher expression in the osteoarthritic group (P = 0.011, P = 0.001, respectively), whereas RANKL expression and the ratio RANKL/OPG were both significantly lower in the osteoarthritic group than in the fracture group. Expression of cathepsin K, MMP-9, and TRAP relative to RANKL was significantly higher in the osteoarthritic group. Ratios of all three proteolytic enzymes relative to formation marker osteocalcin were higher in the fracture group. Gene expression of cathepsin K, MMP-9, TRAP, RANKL, OPG, and osteocalcin and the association between their mRNA levels pointed to higher bone resorption and bone formation in osteoarthritis, differences in balance between them, and differences in regulation of bone resorption in osteoarthritic and osteoporotic bone.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, SI-1000, Ljubljana, Slovenia

    Darja Bitenc Logar, Barbara Ostanek, Zoran Trošt & Janja Marc

  2. Department of Traumatology, General and Teaching Hospital Celje, Celje, Slovenia

    Radko Komadina

  3. Department of Endocrinology and Metabolic Diseases, Clinical Centre Ljubljana, Ljubljana, Slovenia

    Janez Preželj

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  1. Darja Bitenc Logar
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  2. Radko Komadina
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  3. Janez Preželj
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  4. Barbara Ostanek
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  5. Zoran Trošt
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  6. Janja Marc
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Correspondence to Janja Marc.

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Logar, D., Komadina, R., Preželj, J. et al. Expression of bone resorption genes in osteoarthritis and in osteoporosis. J Bone Miner Metab 25, 219–225 (2007). https://doi.org/10.1007/s00774-007-0753-0

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  • DOI: https://doi.org/10.1007/s00774-007-0753-0

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