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Correlations between both the expression levels of inflammatory mediators and growth factor in medial perimeniscal synovial tissue and the severity of medial knee osteoarthritis

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Abstract

An enhanced expression of the inflammatory mediators in the perimeniscal synovium in knee osteoarthritis (OA) has been suggested to contribute to progressive cartilage degeneration. However, whether the expression levels of these molecules correlated with the severity of OA still remained unclear. Medial perimeniscal synovial samples were obtained from 23 patients with Kellgren-Lawrence (K/L) grades 2 to 4 of medial knee OA. Immunohistochemical analysis of the synovium revealed that the MMP-1, COX-2 and IL-1β expression of the patients with K/L 4 to be significantly reduced in comparison to those with either K/L 2 or 3, while the TGF-β expression showed the opposite. The synovial expression of MMP-1 and IL-1β showed a significant negative correlation with the severity of OA, while that of TGF-β again showed the opposite. In conclusion, although synovial inflammation remained active, the MMP-1, COX-2 and IL-1β expression in synovium decreased depending upon the severity of OA, while the TGF-β expression increased.

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Acknowledgement

We wish to thank Dr. Takayuki Kawasaki, Dr. Hiroshi Ikeda, Dr. Yuji Takazawa, Dr. Yoshitomo Saita, and Dr. Yuta Kimura for their helpful comments.

This study was partially supported by grants from the High Technology Research Center Grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and from the Takeda Science Foundation (to M.I.).

Conflict of interest

All authors report no conflicts of interest.

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Authors and Affiliations

  1. Department of Medicine for Motor Organ, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Liang Ning, Muneaki Ishijima, Haruka Kaneko, Lizu Liu, Zhuo Xu, Ippei Futami, Anwarjan Yusup, Kazuo Kaneko & Hisashi Kurosawa

  2. Department of Orthopaedics, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan

    Muneaki Ishijima, Haruka Kaneko, Mitsuaki Kubota, Ippei Futami, Shouyu Xu, Kazuo Kaneko & Hisashi Kurosawa

  3. Sportology Center, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Muneaki Ishijima & Kazuo Kaneko

  4. Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Hidetake Kurihara

  5. Research Institute for Diseases of Old Ages, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Eri Arikawa-Hirasawa

  6. Division of Biomedical Imaging Research, Biomedical Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan

    Katsumi Miyahara

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  1. Liang Ning
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  2. Muneaki Ishijima
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  7. Lizu Liu
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  8. Zhuo Xu
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  11. Katsumi Miyahara
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  12. Shouyu Xu
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  13. Kazuo Kaneko
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  14. Hisashi Kurosawa
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Corresponding author

Correspondence to Muneaki Ishijima.

Additional information

Liang Ning, Muneaki Ishijima and Haruka Kaneko contributed equally to this study.

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Ning, L., Ishijima, M., Kaneko, H. et al. Correlations between both the expression levels of inflammatory mediators and growth factor in medial perimeniscal synovial tissue and the severity of medial knee osteoarthritis. International Orthopaedics (SICOT) 35, 831–838 (2011). https://doi.org/10.1007/s00264-010-1045-1

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  • DOI: https://doi.org/10.1007/s00264-010-1045-1

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