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Association of the HLA region with multiple sclerosis as confirmed by a genome screen using >10,000 SNPs on DNA chips

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Abstract

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system, with a complex genetic background. Here, we present a genome screen for association in small scale, employing 11,555 single nucleotide polymorphisms (SNPs) on DNA chips for genotyping 100 MS patients stratified for HLA-DR2+ and 100 controls. More than 500 SNPs revealed significant differences between cases and controls before Bonferroni correction. A fraction of these SNPs was reanalysed in two additional cohorts of patients and controls, using high-throughput genotyping methods. A marker on chromosome 6p21.32 (rs2395182) yielded the highest significance level, validating the established HLA-DR association.

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Acknowledgements

This study was supported by the Hertie Stiftung (Frankfurt, Germany, project no. 1.319.110/02/05) and the German Federal Ministry of Science and Education through the National Genome Research Network (NGFN grant 01GR0104). The skilful technical assistance of A. Petzold and K. Schlang are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Human Genetics, Ruhr-University, 44780, Bochum, Germany

    René Gödde & Jörg T. Epplen

  2. Gene Mapping Centre (GMC), Max Delbrück Centre for Molecular Medicine, Berlin, Germany

    Klaus Rohde, Christian Becker, Mahammad R. Toliat, Patricia Entz, Anita Suk & Peter Nürnberg

  3. Department of Transfusion Medicine, Universitätsklinikum Essen, Essen, Germany

    Norbert Müller

  4. Department of Neurology, Kliniken Bergmannsheil, Ruhr-University, Bochum, Germany

    Eckhart Sindern

  5. Department of Neurology, Knappschaftskrankenhaus, Ruhr-University, Bochum, Germany

    Michael Haupts

  6. Department of Neurology, St. Josef-Hospital, Ruhr-University, Bochum, Germany

    Sebastian Schimrigk

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  1. René Gödde
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  2. Klaus Rohde
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  3. Christian Becker
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Correspondence to René Gödde.

Additional information

P. Nürnberg and J.T. Epplen contributed equally.

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Gödde, R., Rohde, K., Becker, C. et al. Association of the HLA region with multiple sclerosis as confirmed by a genome screen using >10,000 SNPs on DNA chips. J Mol Med 83, 486–494 (2005). https://doi.org/10.1007/s00109-005-0650-8

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  • DOI: https://doi.org/10.1007/s00109-005-0650-8

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