Abstract
We adapted the method of99mTc-pertechnetate (99mTc) uptake measurements to the mouse knee for detection and quantification of arthritis because clinical assessment of mouse knee-joint arthritis is not reliable. The main points to ensure reproducibility of measurements were proper fixation and positioning of the knee and careful shielding of the rest of the body from the gamma-radiation detector.99mTc uptake was calculated as the mean of three countings. The variation coefficient of these countings ranged from 0.007 to 0.082 in non-arthritic joints and from 0.025 to 0.081 in arthritic joints. Arthritis was scored as the ratio of the99mTc uptake in the right knee versus that in the left knee. This ratio averated 1.06 (S.D. 0.05) in non-arthritic mice 20 minutes after99mTc administration i.p. On the second day after induction of arthritis in the right knee, this ratio ranged from 1.44 to 1.96; this was significantly higher (P<0.005) than in non-arthritic mice, and the increase remained significant during at least 20 days.99mTc uptake measurements seem to be a useful method to detect and quantify arthritis of the knee joint in mice.
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References
D. Brackertz, G.F. Mitchell andI.R. Mackay,Antigen-induced arthritis in mice. I. Induction of arthritis in various strains of mice, Arthritis Rheum.20, 841–850 (1977).
E.C. Keystone, H.U. Schorlemmer, C. Pope andA.C. Allison Zymosan-induced arthritis. A model of chronic proliferative arthritis following activation of the alternative pathway of complement, Arthritis Rheum.20, 1396–1401 (1977).
W.B. van den Berg, M.W.M. Kruijsen andL.B.A. van de Putte, In vivo studies on the influence of antigen-induced joint inflammation on hyaline articular cartilage. InInflammation: Mechanisms and Treatment, pp. 389–394. (EdsD.A. Willoughby andJ.P. Giroud). MTP Press Ltd, London 1980.
A.M.Th. Boerbooms andW.C.A.M. Buys,Rapid assessment of 99m Tc-pertechnetate uptake in the knee-joint as a parameter of inflammatory activity, Arthritis Rheum.21, 348–352 (1978).
H.J. van Beusekom, W.J.M. van den Broek, L.B.A. van de Putte, W.C.A.M. Buys andW.B. van den Berg,99m Tc-pertechnetate uptake measurements in the rabbit knee-joint. I. Standardization of the method and measurements in experimental arthritis, J. Rheumatol.8, 31–39 (1981).
T.D.V. Cooke andM. Maeda,The role of systemic antigen on chronic antigen-induced arthritis in rabbits, ARA abs. Arthritis Rheum.23, 663 (1980).
M.W.M. Kruijsen, W.B. van den Berg, H.J. van Beusekom andL.B.A. van de Putte,Exacerbation of antigen-induced arthritis after heterotopic antigen injection. In preparation.
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Kruijsen, M.W.M., van den Berg, W.B., van de Putte, L.B.A. et al. Detection and quantification of experimental joint inflammation in mice by measurement of99mTc-pertechnetate uptake. Agents and Actions 11, 640–642 (1981). https://doi.org/10.1007/BF01978775
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DOI: https://doi.org/10.1007/BF01978775