Abstract
The pyridinium crosslinks are important and definite biomarkers of mature hard tissue collagen degradation. A gradient ion-paired reversed-phase high-performance liquid chromatographic method was used for the simultaneous determination of both crosslinks in synovial fluid (SF) samples of 25 patients with rheumatoid arthritis (RA). The mean±SD levels of pyridinoline (Pyd) and deoxypyridinoline (Dpyd) in SF were 107.7±182.3 nmol/1 and 4.8±8.3 nmol/1, respectively. The Pyd/Dpyd ratio, which indicates the amount of Pyd released from cartilage rather than bone, amounted to 30.8±29.5. This value is significantly higher than in urine or serum of the same patients. These data suggest increased destruction of joint cartilage in patients with RA and the release of collagen II fragments in SF. In addition, the levels of the crosslinks in SF reflect considerable interindividual variation, indicating substantial individual differences in the amount of collagenous material that is degraded.
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Seibel MJ, Zipf A, Ziegler R (1994) Pyridinium-Crosslinks im Urin. Dtsch Med Wochenschr 119:923–929
Kongtawelert P, Ghosh P (1990) A new sandwich-ELISA method for the determination of keratan sulphate peptides in biological fluids employing a monoclonal antibody and labelled avidin biotin technique. Clin Chim Acta 195:17–26
Spector TD, James IT, Hall GM, Thompson PW, Perrett D, Hart DJ (1993) Increased levels of urinary collagen crosslinks in females with rheumatoid arthritis. Clin Rheumatol 12:240–244
Campion GV, McGrea TJ, Schnitzer TJ (1991) Levels of keratan sulfate in the serum and synovial fluid of patients with osteoarthritis of the knee. Arthritis Rheum 34:1254–1259
Kleesieck K, Reinards R, Okusi J (1987) MDP-β-Xylosyltrans-ferase: anew marker of cartilage destruction in chronic joint diseases. J Clin Chem Clin Biochem 25:473–481
Mollenhauer J, Müller N, Thonar EJ (1990) Neue Ansätze in der Laboratoriumsdiagnostik der Osteoarthritis. Orthopäde 19:28–35
Fife RS, Rachow JW, Ryan LM (1994) Synovial fluid and plasma levels of cartilage matrix glycoprotein in arthritis. Calcif Tissue Int 55:100–102
Mansson B, Carey D, Alini M, Ionescu M, Rosenberg LC, Poole AR, Heinegard D, Saxne T (1995) Cartilage and bone metabolism in rheumatoid arthritis. Differences between rapid and slow progression of disease identified by serum markers of cartilage metabolism. J Clin Invest 95:1071–1077
Poole AR, Dieppe P (1994) Biological markers in rheumatoid arthritis. Semin Arthritis Rheum 23 [Suppl 2]:17–31
Eyre DR, Paz MA, Gallop PM (1984) Cross-linking in collagen and elastin. Ann Rev Biochem 53:717–748
Robins SP (1983) Cross-linking of collagen. Isolation, structural characterization and glycosylation of pyridinoline. Biochem J 215:167–173
Eyre DR, Koob TJ, Van Ness KP (1984) Quantitation of hydroxypyridinium crosslinks in collagen by high-performance liquid chromatography. Anal Biochem 137:380–388
Robins SP, Duncan A (1983) Cross-linking of collagen. Location of pyridinoline in bovine articular cartilage at two sites of the molecule. Biochem J 215:175–182
Robins SP, Duncan A, Riggs BL (1990) In: Christiansen C, Overgaard K (eds) Osteoporosis. Osteopress, Copenhagen, pp 465–468
Black D, Duncan A, Robins SP (1988) Quantitative analysis of the pyridinium crosslinks of collagen in urine using ion-paired reversed-phase high-performance liquid chromatography. Anal Biochem 169:197–203
Pratt DA, Daniloff Y, Duncan A, Robins SP (1992) Automated analysis of the pyridinium crosslinks of collagen in tissue and urine using solid-phase extraction and reversed-phase high-performance liquid chromatography. Anal Biochem 207:168–175
Hein G, Müller A, Franke S, Moth M, Spots E (1995) Study on the correlation of the pyridinium crosslinks (measured in urine and serum) in rheumatoid arthritis. Aktuelle Rheumatol 20:140–144
Abbiati G, Rigoldi M, Frignani S, Colombo L, Mussini E (1994) Determination of pyridinium crosslinks in plasma and serum by high-performance liquid chromatography. J Chromatogr B Biomed Appl 656:303–310
Takahashi M, Kushida K, Ohishi T, Kawana K, Hoshino H, Uchiyama A, Inoue T (1994) Quantitative analysis of crosslinks pyridinoline and pentosidine in articular cartilage of patients with bone and joint disorders. Arthritis Rheum 37:724–728
Ricardblum S, Chevalier X, Grimaud JA, Largetpiet B, Uebelhart D (1995) Detectable levels of pyridinoline are present in synovial fluid from various patients with knee effusion: preliminary results. Eur J Clin Invest 25:438–441
Sinigaglia L, Varenna M, Binelli L, Bartucci F, Arrigoni M, Ferrara R, Abbiati G (1995) Urinary and synovial pyridinium crosslink concentrations in patients with rheumatoid arthritis and osteoarthritis. Ann Rheum Dis 54:144–147
Colwell A, Russell RG, Eastell R (1993) Factors affecting the assay of urinary 3-hydroxy pyridinium crosslinks of collagen as markers of bone resorption. Eur J Clin Invest 23:341–349
James I, Crowley C, Perrett D (1993) Assay of pyridinium crosslinks in serum using narrow-bore ion-paired reversed-phase high-performance liquid chromatography. J Chromatogr 612:41–48
Geiler G, Stiehl P (1974) Zur Bedeutung der Begriffe Basisaktivität und aktuelle Aktivität in der morphologischen Beurteilung der Synovialmembran bei Rheumatoid-Arthritis. Z Rheumatol 33:73–86
Larsen A, Dale K, Eek M (1977) Radiographic evaluation of rheumatoid arthritis and related conditions by standard reference films. Acta Radiol Diagn 18:481–491
Robins SP, McLaren A, Nicol P (1992) Pyridinium cross-links measurements in serum and synovial fluid of patients with osteoarthritis. In: Kuettner KE, Schleyerbach R, Peyron JG, Hascall VC (eds) Articular cartilage and osteoarthritis. Raven Press, New York, pp 738–739
Westacott CI, Whicher JT, Barnes IC, Thompson D, Swan AJ, Dieppe PA (1990) Synovial fluid concentration of five different cytokines in rheumatic diseases. Ann Rheum Dis 49:676–681
Pratta MA, Di Meo TM, Ruhl DM, Arner EC (1989) Effect of interleukin-1-β and tumour necrosis factor-α on cartilage proteoglycan metabolism in vitro. Agents Actions 27:250–253
Wascher TC, Herrmann J, Brezinschek R, Brezinschek HP, Wilders-Truschnig M, Rainer F (1994) Serum levels of interleukin-6 and tumour-necrosis-factor-alpha are not correlated to disease activity in patients with rheumatoid arthritis after treatment with low-dose methotrexate. Eur J Clin Invest 24:73–75
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Müller, A., Hein, G., Franke, S. et al. Quantitative analysis of pyridinium crosslinks of collagen in the synovial fluid of patients with rheumatoid arthritis using high-performance liquid chromatography. Rheumatol Int 16, 23–28 (1996). https://doi.org/10.1007/BF01419951
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DOI: https://doi.org/10.1007/BF01419951