Summary
The effects of recombinant human interleukin-4 (IL-4) and the glucocorticoid, dexamethasone, on tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) levels in cultures of rheumatoid and osteoarthritic synovial tissue were studied. Low concentrations of IL-4 and dexamethasone suppressed the levels of both cytokines in the supernatants of both types of tissue after stimulation with lipopolysaccharide (LPS); the IL-1β and TNFα levels were measured by ELISA. It is suggested that it is the monocyte/macrophage in the synovial tissues that is responsive to the inhibitors. It is proposed that glucocorticoids may act on synovial tissue in this manner in vivo and IL-4 may do so if administered intraarticularly.
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Lipsky PE, Davis LS, Cush JJ, Oppenheimer-Marks N (1989) The role of cytokines in the pathogenesis of rheumatoid arthritis. Springer Semin Immunopathol 11:123–162
Dayer J-M, de Rochemonteix B, Burrus B, Demczuk S, Dinarello C (1986) Human recombinant interleukin-1 stimulates collagenase and prostaglandin E2 production by human synovial cells. J Clin Invest 77:645–648
Leizer T, Clarris BJ, Ash PE, van Damme J, Saklatvala J, Hamilton JA (1987) Interleukin-1β and interleukin-1α stimulate the plasminogen activator activity and prostaglandin E2 levels of human synovial cells. Arthritis Rheum 30:562–566
Butler DM, Piccoli DS, Hart PH, Hamilton JA (1988) Stimulation of human synovial fibroblast DNA synthesis by recombinant human cytokines. J Rheumatol 15:1463–1470
Campbell IK, Piccoli DS, Butler DM, Singleton DK, Hamilton JA (1988) Recombinant human interleukin-1 stimulates human articular cartilage to undergo resorption and human chondrocytes to produce both tissue- and urokinase-type plasminogen activator. Biochim Biophys Acta 967:183–194
Campbell IK, Piccoli DS, Hamilton JA (1990) Stimulation of human chondrocyte prostaglandin E2 production by recombinant human interleukin-1 and tumor necrosis factor. Biochim Biophys Acta 1051:310–318
Campbell IK, Piccoli DS, Roberts MJ, Muirden KD, Hamilton JA (1990) Effects of tumor necrosis factor α and β on resorption of human articular cartilage and production of plasminogen activator by human articular chondrocytes. Arthritis Rheum 33:542–552
Pettipher ER, Higgs GA, Henderson B (1986) Interleukin-1 induces leukocyte infiltration and cartilage proteoglycan degradation in the synovial joint. Proc Natl Acad Sci USA 83:8749–8753
Yocum DE, Esparza L, Dubry S, Benjamin JB, Volz R, Scuderi P (1989) Characteristics of tumor necrosis factor production in rheumatoid arthritis. Cell Immunol 122:131–145
Miyasaka N, Sato K, Goto M, Sasano M, Natsuyama M, Inoue K, Nishioka K (1988) Augmented interleukin-1 production and HLA-DR expression in the synovium of rheumatoid arthritis patients. Possible involvement in joint destruction. Arthritis Rheum 31:480–486
Fiestein GS, Zvaifler NJ (1990) How important are T cells in chronic rheumatoid synovitis? Arthritis Rheum 33:768–773
Hart PH, Vitti GF, Burgess DR, Whitty GA, Piccoli DS, Hamilton JA (1989) Potential anti-inflammatory effects of interleukin-4: Suppression of human monocyte tumor necrosis factor α, interleukin-1, and prostaglandin E2. Proc Natl Acad Sci USA 86:3803–3807
Cheung DL, Hart PH, Vitti GF, Whitty GA, Hamilton JA (1990) Contrasting effects of interferon-gamma and interleukin-4 on the interleukin-6 activity of stimulated human monocytes. Immunology 71:70–75
Miossec P, Naviliat M, D'Angeac AD, Sany J, Banchereau J (1990) Low levels of interleukin-4 and high levels of transforming growth factor β in rheumatoid synovitis. Arthritis Rheum 33:1180–1187
Ropes MW, Bennett GA, Cobb S, Jacox R, Jesser RA (1958) Revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis 9:175–176
Meager A, Parti S, Leung H, Peel E, Mahon B (1987) Preparation and characterisation of monoclonal antibodies directed against antigenic determinants of recombinant human tumor necrosis factor (rTNF). Hybridoma 6:305–311
Kirkham BW, Navarro FJ, Korkill MM, Barbatisc C, Panayi GS (1989) Immunohistochemical localization of interleukin-1 in rheumatoid and osteoarthritis synovial membrane. Br J Rheumatol 28 [Suppl]:47
Ruschen S, Lemm G, Warnatz H (1989) Spontaneous and LPS-stimulated production of intracellular IL-1β by synovial macrophages in rheumatoid arthritis is inhibited by IFN-γ. Clin Exp Immunol 76:246–251
Danis VA, March LM, Nelson DS, Brooks PA (1987) Interleukin-1 secretion by peripheral blood monocytes and synovial macrophages from patients with rheumatoid arthritis. J Rheumatol 14:33–39
Brown MF, Hazlemann BL, Dingle JT, Dandy DG, Murley AHG (1987) Production of cartilage degrading activity by human synovial tissues. Ann Rheum Dis 46:319–323
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Bendrups, A., Hilton, A., Meager, A. et al. Reduction of tumor necrosis factor α and interleukin-1β levels in human synovial tissue by interleukin-4 and glucocorticoid. Rheumatol Int 12, 217–220 (1992). https://doi.org/10.1007/BF00301004
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DOI: https://doi.org/10.1007/BF00301004