Summary
Platelets were isolated by gel filtration from paired samples of peripheral blood and synovial fluid (SF) aspirated from inflamed knee joints from 20 adult patients with rheumatoid arthritis (RA) as well as from peripheral blood obtained from 20 healthy subjects. The platelets from the three different sources were investigated for quantitative differences in the number of two distinct types of intracellular storage organelles using immunofluorescence staining for platelet factor 4 (PF4) and labelling with the fluorescent substance mepacrine (MC). The number of PF4-stained organelles per cell was the same in the peripheral normal and RA platelets. This number was distinctly lower in the SF platelets. The peripheral and SF platelets from the RA patients had the same number of MC-labelled organelles. This number was distinctly lower than in the normal cells. The results suggest that the peripheral RA platelets had been activated to liberate serotonin and other substances from one type of organelles, and that the SF platelets had been activated to an additional liberation of PF4 from another such type. Liberated PF4, serotonin, and other substances from SF platelets may, in several ways, contribute to the inflammatory responses of RA.
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References
Kaplan KL (1981) Platelet granule proteins. In: Gordon JL (ed) Localization and secretion, vol 2. Elsevier, Amsterdam, pp 77–90
Ginsberg MH, Taylor L, Painter RG (1980) The mechanism of thrombin-induced platelet factor 4 secretion. Blood 55:661–668
Ginsberg MH, Breth G, Skosey JL (1978) Platelets in the synovial space. Arthritis Rheum 21:994–995
Yaron M, Djaldetti M (1978) Platelets in synovial fluid. Arthritis Rheum 21:607–608
Endresen GKM (1981) Investigation of blood platelets in synovial fluid from patients with rheumatoid arthritis. Scand J Rheumatol 10:204–208
Endresen GKM (1984) Demonstration of fibronectin associated with platelets in synovial fluid from patients with rheumatoid arthritis. Scand J Rheumatol 13:351–356
Kaplan KL, Broekman MJ, Chernoff A, Lesznik GR, Drillings M (1979) Platelet α-granule proteins: studies on release and subcellular localization. Blood 53:604–618
Hiti-Harper J, Wohl H, Harper E (1978) Platelet factor 4: an inhibitor of collagenase. Science 199:991–992
Lonky SA, Marsh J, Wohl H (1978) Stimulation of human granulocyte elastase by platelet factor 4 and heparin. Biochem Biophys Res Commun 85:1113–1118
Aalto M, Kulonen E (1972) Effects of serotonin, indomethacin and other antirheumatic drugs on the synthesis of collagen and other proteins in granulation tissue slices. Biochem Pharmacol 21:2835–2840
Deuel TF, Senior RM, Chang D, Griffin GL, Heinrikson RL, Kaiser ET (1981) Platelet factor 4 is chemotactic for neutrophils and monocytes. Proc Natl Acad Sci USA 78:4584–4587
Yeatts RP, Turner R, Collins R, Kaufmann J, Mashburn H (1978) Soluble and insoluble immune complex-platelet interactions in rheumatoid inflammation. Ann Rheum Dis 34:421–427
Ropes MW, Bennett GA, Cobb S, Jacox R, Jessar RA (1958) 1958 Revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis 9:175–176
Førre Ø, Michaelsen TE, Natvig JB (1976) Antibody activity of heavy and light chains and recombined IgG of human IgG anti-D. Scand J Immunol 5:155–160
Boneu B, Caranobe C, Capdeville J, Robert A, Bierme R (1978) Quantitative evaluation of mepacrine labelled human platelet dense bodies in normals and in cases of peripheral thrombocytopenia. Thromb Res 12:831–839
Endresen GKM, Førre Ø (1982) Studies on the binding of immunoglobulins and immune complexes to the surface of human platelets: IgG molecules react with platelet Fc receptors with the CH3 domain. Int Archs Allergy appl Immunol 67:33–39
Little CH, Stewart AG, Fennessy MR (1983) Platelet serotonin release in rheumatoid arthritis: a study in food-intolerant patients. Lancet II:297–299
Zeller J, Weissbarth E, Baruth B, Mielke H, Deicher H (1983) Serotonin content of platelets in inflammatory rheumatic diseases. Correlation with clinical activity. Arthritis Rheum 26:532–540
Myers SL, Christine TA (1982) Measurement of betathromboglobulin connective tissue activating peptide-III platelet antigen concentrations in pathologic synovial fluids. J Rheumatol 9:6–12
Fink PC, Piening U, Fricke M, Deicher H (1979) Platelet aggregation and aggregation inhibition by different antiglobulins and antiglobulin complexes from sera of patients with rheumatoid arthritis. Arthritis Rheum 22:896–903
Valone FH, Austen KF, Goetzl EJ (1979) Identification of the platelet activating activity in rheumatoid synovial fluid as an intermediate molecular weight complex of IgG. J Immunol 122:703–709
Shapleigh C, Valone FH, Schur PH, Goetzl EJ, Austen KF (1980) Platelet-activating activity in synovial fluids of patients with rheumatoid arthritis, juvenile rheumatoid arthritis, gout, and noninflammatory arthropathies. Arthritis Rheum 23:800–807
Weissbarth E, Baruth B, Mielke H, Liman W, Deicher H (1982) Platelets as target cells in rheumatoid arthritis and systemic lupus erythematosus: a platelet specific immunoglobulin inducing the release reaction. Rheumatol Int 2:67–73
Balleisen L, Gay S, Marx R, Kühn K (1975) Comparative investigation on the influence of human and bovine collagen types I, II and III on the aggregation of human platelets. Klin Wochenschr 53:903–905
Panayi GS, Johnson PM (1979) Immunopathogenesis of rheumatoid arthritis. Proceedings of the international symposium held at Guy's Hospital, London, November 1978. Reedbooks, Chertsey, Surrey, England
Huijgens PC, van den Berg CAM, Imandt LMFM, Langenhuijsen MMAC (1981) Sulphinpyrazone and indomethacin for inhibition of platelet prostaglandin synthesis. Thromb Res 24:267–270
Prowse C, Pepper D, Dawes J (1982) Prevention of the platelet alpha-granule release reaction by membrane-active drugs. Thromb Res 25:219–227
Meyerhoff J, Dorsch CA (1981) Decreased platelet serotonin levels in systemic lupus erythematosus. Arthritis Rheum 24:1495–1500
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Endresen, G.K.M. Evidence for activation of platelets in the synovial fluid from patients with rheumatoid arthritis. Rheumatol Int 9, 19–24 (1989). https://doi.org/10.1007/BF00270285
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DOI: https://doi.org/10.1007/BF00270285