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Mesenchymal Stem Cell Assays and Applications pp 295–303Cite as

Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 698))

Abstract

The generation of dopamine (DA) neurons from stem cells holds great promise for future biomedical research and in the clinical treatment of neurodegenerative diseases, such as Parkinson’s disease. Mesenchymal stem cells (MSCs) derived from the adult human bone marrow (BM) can be easily isolated and expanded in culture while maintaining their immense plasticity. Here, we describe a protocol to generate DA-producing cells from adult human MSCs using a cocktail that includes sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), and basic fibroblast growth factor (bFGF). Electrophysiological functional DA neurons could be achieved by further treatment with brain-derived neurotrophic factor (BDNF). In summary, a protocol is described for the induction of primary BM-derived human MSCs to specific transdifferentiation; in this case, functional DA neurons. The MSC-derived DA cells express DA-specific markers, synthesize, and secrete dopamine. The described method could be used to generate DA cells for various model systems in which DA-producing cells are implicated in pathophysiological conditions.

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References

  1. Picinich, S.C., Mishra, P.J., Mishra, P.J., Glod, J., Banerjee, D. (2007) The therapeutic potential of mesenchymal stem cells. Cell- & tissue-based therapy. Expert Opin Biol Ther 7, 965–73.

    Article  PubMed  CAS  Google Scholar 

  2. Zipori, D. (2004) Mesenchymal stem cells: harnessing cell plasticity to tissue and organ repair. Blood Cells Mol Dis 33, 211–5.

    Article  PubMed  CAS  Google Scholar 

  3. Giordano, A., Galderisi, U., Marino, I.R. (2007) From the laboratory bench to the patient’s bedside: an update on clinical trials with mesenchymal stem cells. J Cell Physiol 211, 27–35.

    Article  PubMed  CAS  Google Scholar 

  4. Phinney, D.G., Isakova, I. (2005) Plasticity and therapeutic potential of mesenchymal stem cells in the nervous system. Curr Pharm Des 11, 1255–65.

    Article  PubMed  CAS  Google Scholar 

  5. Bianco, P., Riminucci, M., Gronthos, S., Robey, P.G. (2001) Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells 19, 180–92.

    Article  PubMed  CAS  Google Scholar 

  6. Potian, J.A., Aviv, H., Ponzio, N.M., Harrison, J.S., Rameshwar, P. (2003) Veto-like activity of mesenchymal stem cells: functional discrimination between cellular responses to alloantigens and recall antigens. J Immunol 171, 3426–34.

    PubMed  CAS  Google Scholar 

  7. Krampera, M., Pasini, A., Pizzolo, G., Cosmi, L., Romagnani, S., Annunziato, F. (2006) Regenerative and immunomodulatory potential of mesenchymal stem cells. Curr Opin Pharmacol 6, 435–41.

    Article  PubMed  CAS  Google Scholar 

  8. Greco, S.J., Zhou, C., Ye, J.H., Rameshwar, P. (2007) An interdisciplinary approach and characterization of neuronal cells transdifferentiated from human mesenchymal stem cells. Stem Cells Dev 16, 811–26.

    Article  PubMed  CAS  Google Scholar 

  9. Tropel, P., Platet, N., Platel, J.C., Noel, D., Albrieux, M., Benabid, A.L., Berger, F. (2006) Functional neuronal differentiation of bone marrow-derived mesenchymal stem cells. Stem Cells 24, 2868–76.

    Article  PubMed  CAS  Google Scholar 

  10. Sato, Y., Araki, H., Kato, J., Nakamura, K., Kawano, Y., Kobune, M., Sato, T., Miyanishi, K., Takayama, T., Takahashi, M., Takimoto, R., Iyama, S., Matsunaga, T., Ohtani, S., Matsuura, A., Hamada, H., Niitsu, Y. (2005) Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion. Blood 106, 756–63.

    Article  PubMed  CAS  Google Scholar 

  11. Zeng, X., Cai, J., Chen, J., Luo, Y., You, Z.B., Fotter, E., Wang, Y., Harvey, B., Miura, T., Backman, C., Chen, G.J., Rao, M.S., Freed, W.J. (2004) Dopaminergic differentiation of human embryonic stem cells. Stem Cells 22, 925–40.

    Article  PubMed  CAS  Google Scholar 

  12. Lindvall, O., Kokaia, Z., Martinez-Serrano, A. (2004) Stem cell therapy for human neurodegenerative disorders-how to make it work. Nat Med 10 Suppl, S42–50.

    Article  PubMed  Google Scholar 

  13. Correia, A.S., Anisimov, S.V., Li, J.Y., Brundin, P. (2005) Stem cell-based therapy for Parkinson’s disease. Ann Med 37, 487–98.

    Article  PubMed  CAS  Google Scholar 

  14. Trzaska, K.A., Kuzhikandathil, E.V., Rameshwar, P. (2007) Specification of a dopaminergic phenotype from adult human mesenchymal stem cells. Stem Cells 25, 2797–808.

    Article  PubMed  CAS  Google Scholar 

  15. Trzaska, K.A., King, C.C., Le, K.Y., Kuzhikandathil, E.V., Nowycky, M.C., Ye, J.H., Rameshwar, P. (2009) Functional maturation of mesenchymal stem cell-derived dopamine progenitors by brain-derived neurotrophic factor. J Neurochem 110, 1058–69.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work has been supported by the F.M. Kirby Foundation.

Author information

Authors and Affiliations

  1. Department of Medicine Hematology/Oncology, University of Medicine and Dentistry of New Jersey – New Jersey Medical School, 185 South Orange Avenue, Newark, NJ, USA

    Pranela Rameshwar

Authors
  1. Katarzyna A. Trzaska
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  2. Pranela Rameshwar
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Corresponding author

Correspondence to Pranela Rameshwar .

Editor information

Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Frederick, 21704, USA

    Mohan Vemuri

  2. Cellular Dynamics International, Inc., Science Drive 525, Madison, 53711, Wisconsin, USA

    Lucas G. Chase

  3. Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mahendra S. Rao

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© 2011 Springer Science+Business Media, LLC

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Trzaska, K.A., Rameshwar, P. (2011). Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells. In: Vemuri, M., Chase, L., Rao, M. (eds) Mesenchymal Stem Cell Assays and Applications. Methods in Molecular Biology, vol 698. Humana Press. https://doi.org/10.1007/978-1-60761-999-4_22

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  • DOI: https://doi.org/10.1007/978-1-60761-999-4_22

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-998-7

  • Online ISBN: 978-1-60761-999-4

  • eBook Packages: Springer Protocols

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