C-REACTIVE PROTEIN AND SERUM AMYLOID A mRNA STABILITY FOLLOWING INDUCTION BY CYTOKINES

doi:10.1006/cyto.1996.0072

Cytokine

Volume 8, Issue 7, July 1996, Pages 534-540

https://doi.org/10.1006/cyto.1996.0072 Get rights and content

Abstract

We determined the effects of cytokine withdrawal on C-reactive protein (CRP) and serum amyloid A (SAA) mRNA abundance in Hep3B cells following 24 h of preinduction with interleukin 6 plus interleukin 1β. After cytokine withdrawal, CRP transcription rate rapidly fell to undetectable levels and mRNA levels fell with a half-disappearance time of about 2.5 h. In view of the relatively small amount of CRP transcription occurring at this time, it is likely that this value closely re?ects the actual half-life of CRP mRNA. In contrast, substantial SAA transcription continued for at least 8 h, while SAA mRNA fell with a half-disappearance time of about 8.5 h. It is not possible, under these conditions, to determine SAA mRNA half-life, but it clearly was no greater than 8.5 h. Both Actinomycin D (ActD) and cycloheximide enhanced the stability of SAA mRNA, strongly suggesting that SAA mRNA degradation requires synthesis of a short-lived protein. CRP mRNA stability was also enhanced by ActD, but cycloheximide did not have a protracted stabilizing effect, suggesting complex regulatory processes. These studies provide insight into the stability of CRP and SAA mRNA following induction with [IL-6 + IL-1β] and into the mechanisms regulating their degradation.

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Citation Excerpt :
Such inflammatory conditions, although usually considered clinically insignificant in stranded California sea lions, might theoretically cause release of amyloidogenic cytokines. In blood, SAA has a short half-life (<2 days) and concentrations are therefore increased only during periods of active, systemic inflammation (Lozanski et al., 1996). It has been used as a highly sensitive and specific diagnostic indicator of active generalized inflammation in numerous species, such as dogs, horses, cows and manatees (Trichechus manatus latirostris) (Hulten and Demmers, 2002; Petersen et al., 2004; Harr et al., 2006; Kjelgaard-Hansen et al., 2007).
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Regular ArticleC-REACTIVE PROTEIN AND SERUM AMYLOID A mRNA STABILITY FOLLOWING INDUCTION BY CYTOKINES

Abstract

Regular Article
C-REACTIVE PROTEIN AND SERUM AMYLOID A mRNA STABILITY FOLLOWING INDUCTION BY CYTOKINES