Vγ9-Vδ2+ γδ T Cells from a Patient with Felty Syndrome That Exhibit Aberrant Response to Triggering of the CD3 Molecule Can Regulate Immunoglobulin Secretion by B Cells

doi:10.1006/clin.1995.1024

Clinical Immunology and Immunopathology

Volume 74, Issue 2, February 1995, Pages 162-169

Clinical Immunology ...

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Abstract

Expanded populations of T lymphocytes bearing γδ T cell receptors have been detected in several patients with Felty syndrome. The goal of this study was to functionally characterize these lymphocytes in a newly described patient with this disease. For this, fluorescence-activated cell sorter analysis of T cell surface antigens, proliferation, and tumor necrosis factor α enzyme-linked immunosorbent assays, as well as quantitative assays of immunoglobulins secreted by pokeweed mitogen-driven B cells were performed. The γδ cells, that expressed a CD3⁺CD4^-Vγ9^-Vδ2⁺Cδ⁺ phenotype, and constituted 60% of the peripheral blood T cells, did not proliferate after triggering with anti-CD3, but did secrete tumor necrosis factor α and the addition of these cells to pokeweed mitogen-stimulated B cells from the patient decreased their secretion of immunoglobulin M while augmenting IgG secretion. These data suggest that the expanded anergic Vγ9^-Vδ2⁺ γδ cells can play an immunoregulatory role in the patient.

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Regular ArticleVγ9-Vδ2+ γδ T Cells from a Patient with Felty Syndrome That Exhibit Aberrant Response to Triggering of the CD3 Molecule Can Regulate Immunoglobulin Secretion by B Cells

Abstract

Regular Article
Vγ9^-Vδ2⁺ γδ T Cells from a Patient with Felty Syndrome That Exhibit Aberrant Response to Triggering of the CD3 Molecule Can Regulate Immunoglobulin Secretion by B Cells