Elsevier

Clinical Immunology

Volume 99, Issue 1, April 2001, Pages 82-93
Clinical Immunology

Regular Article
Vitamin D3 and Its Synthetic Analogs Inhibit the Spontaneous in Vitro Immunoglobulin Production by SLE-Derived PBMC

https://doi.org/10.1006/clim.2000.4998Get rights and content

Abstract

The production of high-affinity pathogenic autoantibodies in systemic lupus erythematosus (SLE) may result from aberrant immune regulation. Since 1,25 dihydroxy vitamin D3 (1,25 D3) has immunoregulatory activity, we examined effects of 1,25 D3 and its analogs HM, V, MC1288, and KH1060 on autoantibody production and proliferation of SLE PBMC. We found, in SLE, a higher percentage of T, B, and NK expressing vitamin D3 receptors (VDRs) (P = 0.034, 0.006, 0.012, respectively). Incubating SLE PBMC with 1,25 D3 compounds significantly reduced proliferation, polyclonal and anti-dsDNA IgG production, and the percentages of CD3+/DR+ T and B (CD19+) cells, while elevating NK (CD16+) cells (P < 0.001). 1,25 D3 analogs were more potent than the natural compound: KH1060 up-regulated CD14 expression by SLE monocytes (P < 0.001), inhibited polyclonal and anti-dsDNA IgG production by SLE-derived B lymphoblasts, and induced apoptosis of activated B lymphoblasts. These data suggest that 1,25 D3 compounds can offer novel approaches to the clinical management of SLE.

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    Jim Klinenberg: 6/26/1934–12/28/1999.

    1

    To whom correspondence and reprint requests should be addressed at Cedars-Sinai Medical Center D-5073, 8700 Beverly Blvd., Los Angeles CA 90048. E-mail: [email protected].

    2

    Present address: Humboldt University, Department of Medicine (Charite), Division of Hematology/Oncology, Berlin, Germany.

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