Immunogenic epitopes enriched in dermatomyositis patients with sequence identity between bat coronavirus and SARS-CoV-2
| Bat coronavirus strain/epitope | Immunogenic epitope | Human SARS-CoV-2 sequence identity (YP_009724389.1) | Length | Start-end (AA) | RefSeq protein | Protein |
| BtCoV/279/2005_EP1 | VKGECPVMAPRR | GECP* | 4 | 268–271 | YP_009725298.1 | Non-structural protein 2 |
| BtCoV/279/2005_EP2 | SQAWQPLRQR | SQAWQP† | 6 | 6800–6805 | YP_009725311.1 | 2'-O-ribose methyltransferase |
| LRQ* | 3 | 6883–6885 | YP_009725311.1 | 2'-O-ribose methyltransferase | ||
| BtCoV/279/2005_EP4 | DDAVVCVHGL | DDAVVC† | 6 | 5152–5157 | YP_009725307.1 | RNA-dependent RNA polymerase |
| BtCoV/279/2005_EP5 | GGAVCRERPVS | GGAVCR† | 6 | 6405–6410 | YP_009725309.1 | 3'-to-5' exonuclease |
| Bat_SARS_CoV_Rm1/2004_EP1 | VKGECPVMAPRR | GECP* | 4 | 268–271 | YP_009725298.1 | Non-structural protein 2 |
| Bat_SARS_CoV_Rm1/2004_EP2 | SQAWQPLRQR | SQAWQP† | 6 | 6800–6805 | YP_009725311.1 | 2'-O-ribose methyltransferase |
| LRQ* | 3 | 6883–6885 | YP_009725311.1 | 2'-O-ribose methyltransferase | ||
| Bat_SARS_CoV_Rm1/2004_EP3 | DDAVVCVHGL | DDAVVC† | 6 | 5152–5157 | YP_009725307.1 | RNA-dependent RNA polymerase |
| Bat_SARS_CoV_Rm1/2004_EP4 | GGAVCRERPVS | GGAVCR† | 6 | 6405–6410 | YP_009725309.1 | 3'-to-5' exonuclease |
| Bat_coronavirus_HKU5-5_EP2 | SAGCFVGLPIAG | AGCFV‡ | 4 | 5232–5236 | YP_009725307.1 | RNA-dependent RNA polymerase |
| Bat_coronavirus_HKU5-5_EP3 | WGAVCRKRPVS | GAVCR‡ | 5 | 6406–6410 | YP_009725309.1 | 3'-to-5' exonuclease |
Disease-specific immunogenic epitopes identified against which immunoglobulins were raised. Cross-species alignment carried out using Clustal-O and CLC Genomics Workbench 12.
*Blastp: not available
†Blastp: High specificity for SARS-CoV-2
‡Blastp: Low specificity for SARS-CoV-2
AA, amino acid; SARS-CoV-2, severe acute respiratory syndrome coronavirus-2.