Immunophenotyping results
| Cell subset (gating strategy), count/mm3 | Discovery (n=203) | Validation (n=144) | ||||
| SSc (n=64) | Ctrl (n=139) | q | SSc (n=72) | Ctrl (n=72) | q | |
| Basophils (CD123 HLA-DR−) | 58.3±39.7 | 184.9±464.6 | 0.009 | 44.3±30.6** | 56±60.1* | 0.211 |
| Eosinophils (CD15+ CD16−) | 174.5±371.1 | 167.3±174.8 | NS | 146.6±86.6 | 152.6±95 | NS |
| Neutrophils (CD15− CD16+) | 4116±2389 | 4363.3±2544.1 | NS | 4270±1648.8 | 3312.9±1324.3** | 0.001 |
| LDGs (CD15hi CD14−) | 0±0.1 | 0±0.2 | NS | 0±0.1 | 0±0.1 | NS |
| Classical monocytes (CD14hi CD16−) | 376.9±170.3 | 390.4±220.8 | NS | 342.8±168.8 | 308.8±123.5** | 0.208 |
| Intermediate monocytes (CD14+/hi CD16+) | 52.2±34.8 | 50.9±36.2 | NS | 65.3±37.2** | 48.4±24.8 | 0.004 |
| Non classical monocytes (CD14+ CD16 hi) | 12.3±12.1 | 9.8±7.1 | 0.071 | 10.3±7.6 | 8.2±6.3 | 0.09 |
| NK regulatory (CD3− CD56hi CD16−) | 7±6.4 | 10±7.5 | 0.002 | 4±3.6*** | 6.8±4.6*** | 0.004 |
| NK cytotoxic (CD3− CD56lo CD16hi) | 132.7±95.3 | 234.2±200.9 | 1.18E-05 | 112.4±69.6 | 190.3±121.3 | 0.002 |
| NK-like T cells (CD3+ CD56+) | 65.7±54.9 | 130.9±119.3 | 1.18E-05 | 50.4±74.3** | 101.7±85.1** | 8.45E-05 |
| B cells (CD3− CD19+) | 152±109.9 | 256.3±217.6 | 1.43E-05 | 169.2±136.2 | 191.7±116.6** | 0.212 |
| CD4 T cells (CD3+ CD4+ CD8−) | 649.9±284.3 | 1084.9±893.4 | 1.18E-05 | 607±316 | 760.8±329.7** | 0.043 |
| CD8 T cells (CD3+ CD4 CD8+) | 258.6±144.8 | 408.9±279 | 2.11E-05 | 226.8±154.2 | 304.5±192.2** | 0.044 |
| Double positive T cells (CD3+ CD8+ CD4+) | 11.4±9.5 | 23.1±23.1 | 5.76E-05 | 14.5±20.8 | 17.9±17.8 | 0.16 |
| Double negative T cells (CD3+ CD8- CD4−) | 20.7±14 | 42±33.7 | 1.11E-06 | 22.7±42.6 | 25±20.6*** | 0.185 |
| mDC1 (Lin-HLA-DR+ CD11c+ CD123 lo CD141− CD1c+) | 2.6±3 | 11.3±37.8 | 0.005 | 1.2±1.1** | 3±6.7** | 8.12E-04 |
| mDC2 (Lin-HLA-DR+ CD11c+ CD123 lo CD141+ CD1c−) | 60.1±90.8 | 156±514.6 | 0.046 | 44.7±45.9 | 39.3±37.6** | NS |
| mDC (Lin-HLA-DR+ CD11c +CD123 lo CD141− CD1c) | 17.1±17.2 | 32.1±137.4 | 0.353 | 7.7±8.7* | 10.9±29.9** | NS |
| pDC (Lin-HLA-DR+ CD11c+ CD123 lo CD11c− CD123hi) | 10.2±9.2 | 55.1±174.2 | 8.22E-04 | 4.8±4.9* | 11.2±22.8*** | 2.9E-05 |
False-discovery rate (FDR) corrected p values (q) after cube root transformation to unskew the data.
*q<0.05 versus discovery; **q<0.01 versus discovery; ***q<0.001 versus discovery.
DC, dendritic cells; LDG, low density grains; NK, natural killers.