The five most significant CpGs (white rows) and the five CpGs with the largest effect size (grey rows) from the linear mixed model: DNA methylation in blood samples from children born to mothers with RA (FEPRA study) compared with children born to mothers from the general population (Generation R Study)
| CpG | Beta* | SE | P value | Nearest gene (±bp) | Chr | bp | Location† |
| cg06642177 | 0.028 | 0.002 | 1.32×10−24 | SLC2A12 (−122 529) | 6 | 134 496 341 | – |
| cg08867893 | 0.018 | 0.002 | 7.66×10−24 | ZNF365 (+221) | 10 | 64 134 160 | – |
| cg06778273 | 0.024 | 0.002 | 3.77×10−23 | TNFRSF18 (+4995) | 1 | 1 137 117 | – |
| cg07786668 | 0.026 | 0.002 | 7.11×10−23 | ZFHX3 (−10 142) | 16 | 73 092 391 | – |
| cg20116574 | 0.019 | 0.002 | 5.91×10−19 | NCOA5 (+435) | 20 | 44 718 168 | Promoter |
| cg16930947 | −0.050 | 0.008 | 3.22×10−11 | – | 8 | 88 984 447 | – |
| cg01485645 | −0.044 | 0.006 | 1.64×10−13 | MLLT6 (+303) | 17 | 36 862 199 | Promoter |
| cg12360123 | −0.043 | 0.008 | 1.61×10−08 | – | 10 | 79 984 532 | Enhancer |
| cg06656994 | 0.038 | 0.005 | 9.67×10−13 | FAM163A (+903) | 1 | 179 713 176 | Enhancer |
| cg17483482 | −0.037 | 0.006 | 4.62×10−10 | – | 1 | 117 152 162 | – |
*Beta represents the difference in DNA methylation at the given CpG site in children born to mothers with RA (FEPRA study) as compared with children born to mothers from the general population (Generation R Study).
†Location in promoter, enhancer or unknown (–).
bp, base pair; Chr, chromosome; CpG, cytosine-phosphate-guanine; FEPRA, FEtal Programming in Rheumatoid Arthritis; RA, rheumatoid arthritis.