Diseases and clinical trials | Number of patients, source of cells, dose and route of administration | Outcomes | Comments |
Multiple sclerosis, MS | |||
1. Karussis et al (2010)67
Phase I/II uncontrolled feasibility study of patients with MS and ALS | 34 patients (15 with MS, 19 with ALS) received autologous BM-derived MSCs intrathecally (n=34) at a mean dose of 63.2×106 in 2mls of saline and intravenously (n=14) at a mean dose of 23.4×106 cells in 2mls of saline. | No major AEs. EDSS score improved over 6 months. Proportion of CD4+CD25+ Tregs increased, and expression of CD40, CD83, CD86 and HLA-DR on myeloid dendritic cells decreased 24 hours post-administration. MRI of MSC labelled with superparamagnetic particles showed MSCs in meninges, subarachnoid space, and spinal cord. | No comparison between intravenous and Intrathecal routes as regards homing of MSCs to the CNS. Cryopreserved cells were used. |
2. Bonab et al (2012)68
Phase II uncontrolled study of patients with SPMS | 22 patients received Intrathecal, autologous BM-derived MSCs at a mean dose of 29.5×106 cells in 10mls of normal saline. | AEs were low-grade: transient fever, headache, nausea/vomiting (related to lumbar puncture). Disease progression stabilised in the short-term evidenced by MRI and EDSS score. | After initial improvement some patients reported worsening EDSS, and about 25% showed worsening lesions on MRI, after 12 months. Cryopreservation was not discussed. |
3. Connick et al (2012)69
Phase IIa feasibility/ proof-of-concept study in patients with SPMS | 10 patients received autologous bone marrow (BM)-derived MSCs intravenously at a mean dose of 1.6×106 cells/kg. | Mild AEs such as transient post-transfusion rash and self-limiting bacterial infections. Improvement in visual acuity, visual evoked potentials, optic nerve area and EDSS. No change in post-treatment T cell subset counts. | Cryopreserved cells were used. |
4. Li et al (2014)70
Randomised Controlled Phase II study in patients with RRMS and SPMS | 13 patients received 3 cycles of intravenous, allogeneic umbilical cord (UC)-derived MSCs, 2 weeks apart, at a dose of 4×106 cells/kg body weight in 100mls of saline. Conventional treatment (anti-inflammatory and immunosuppressants) was continued; 10 patients received only conventional treatment. | Reduced frequency of recurrence in the treatment group, who also had a more steady disease course. No significant adverse event. Transient improvement in immunomodulatory cytokines was recorded | Randomised controlled study but not blinded. Cryopreservation was not discussed |
Rheumatoid arthritis | |||
5. Wang et al (2013)72
Phase II non-randomised, controlled study | 172 patients with active RA. 136 received 4×107 allogeneic UC-derived MSCs in 40mls of intravenous saline while 36 received only saline. All patients continued their DMARDS. | No serious adverse events. TNF-alpha and IL-6 decreased while FoxP3+ Tregs increased in the treatment group after infusion. Better clinical outcomes (ACR responses, HAQ and DAS28) after 3 months in the treatment group | Non-randomised study. Treatment group and control group were recruited in different time frames. Cryopreserved cells were used |
6. Alvaro-Gracia et al (2017)73
Dose-escalation, randomised, single-blind (double-blind for efficacy), phase Ib/IIa study | 53 patients with refractory RA (failed two biologics) received three intravenous infusions at different doses (1×106, 2×106 and 4×106 cells/kg) of allogeneic, adipose-derived MSCs or placebo | Generally well-tolerated. Mild adverse events. Dose-dependent response especially DAS28-ESR at 1 month and 3 months post-infusion. Distribution of T cell populations was not significantly modified. | First placebo-controlled study of MSCs in RA. 19% of patients generated mesenchymal stromal cell-specific anti-HLA1 antibodies without apparent clinical consequences. Cryopreserved cells were used |
SLE | |||
7. Sun et al (2009)74
Safety of MSC in Patients with refractory SLE | Four patients with refractory SLE received intravenous, allogeneic BM-derived MSCs at a dose of 1×106 cells/kg. | Safe and well-tolerated. Stable course of SLE disease activity by 12–18 months post-treatment, with improvement in SLEDAI and serological markers. | First study in SLE. Provided evidence for further studies in SLE. Cryopreservation was not discussed. |
8. Liang et al (2010)75
Early phase safety/efficacy study in refractory SLE | 15 patients with refractory SLE were treated with one intravenous infusion of 1×106 cells/kg allogeneic BM-MSC. Mean follow-up period of 17.2 months | No serious adverse events. All patients clinically improved with decrease in SLEDAI, proteinuria, and anti-dsDNA. | Improvement in some patients allowed reduction in doses of steroids and immunosuppressants. Cryopreservation was not discussed. |
9. Sun et al (2010)76
Early phase I/II study | 16 patients with active and refractory SLE on different treatment regimens received 1×106 cells/kg intravenous of UC-derived MSC. | Significant improvement in SLEDAI score, autoantibodies, complement C3 and renal function accompanied by increased Tregs. | Patients clinically improved despite reducing doses of maintenance steroids and immunosuppressants. Cryopreservation was not discussed. |
10. Wang et al (2012)77
Early phase I/II study. Compared the efficacy of single and double infusions | 58 patients with refractory and active SLE. 30 received one intravenous dose of 1×106 cells/kg allogeneic BM-MSCs or UC-MSCs, while 28 received two infusions of 1×106 cells/kg 1 week apart. | No remarkable difference in SLEDAI and serological marker changes between the two groups. | Non-significance of difference in clinical improvement between single and double dose cohorts may be related to sample size. Cryopreservation was not discussed. |
11. Li et al (2013)78
Early phase I/II study in patients with SLE with refractory cytopaenia | 35 patients with SLE with refractory cytopaenia received 1×106 cells/kg of either allogeneic BM-derived or allogeneic UC-derived MSCs and followed up for an average of 21 months. | Well-tolerated. Significant improvement in blood cell counts after MSC treatment. Clinical improvement was also associated with increased Tregs and decreased Th17. | Focused on haematological parameters in SLE. Cryopreservation was not discussed. |
12. Wang et al (2013)79
Early phase I/II 4 year single-centre study | 87 patients with SLE . Allogeneic BM-MSC or UC-MSC infused intravenously at 1×106 cells/kg. Some patients were treated with cyclophosphamide to inhibit active lymphocyte response. 18 patients received repeat doses of MSC for relapses | Generally safe and well-tolerated. SLEDAI score, renal function and blood counts significantly improved for up to 4 years. All patients underwent tapering of steroids and immunosuppressants according to clinical status. | No differences in outcomes between those pretreated with cyclophosphamide and those that were not. No differences with regard to source of cells (UC and BM). Cryopreservation was not discussed. |
13. Wang et al (2014)80
Multicentre phase I/II study | 40 patients with active and refractory SLE received two intravenous doses of 1×106 cells/kg allogeneic UC-derived MSCs while still maintaining baseline immunosuppressants+/-steroids. | Well-tolerated. 60% achieved major clinical response or partial clinical response as determined by BILAG scores. SLEDAI, renal function and serological indices also improved allowing tapering of steroid and immunosuppressant doses. | 12.5% and 16.7% relapse rate at 9 and 12 months, respectively. Cryopreservation was not discussed. |
ACR, American College of Rheumatology; AE: adverse events; ALS, amyotrophic lateral sclerosis; BM, bone marrow; BILAG, British Isles Lupus Activity Group; DAS28, Disease Activity Score-28 joint count; EDSS, Expanded Disability Status Score; HAQ, Health Assessment Questionnaires; RA, rheumatoid arthritis; RRMS, relapsing remitting multiple sclerosis; SLEDAI, Systemic Lupus Erythematosus Disease Activity Index; SPMS,secondarily progressive multiple sclerosis; UC, umbilical cord.