Validation of the differentially expressed miRNAs in T and B cells by quantitative PCR
| Discovery cohort | Replication cohort | Combined analysis | ||||
| miRNAs | Fold change | p Value | miRNAs | p Value | miRNAs | p Value |
| T cells | ||||||
| hsa-let-7d-3p | −2.36 | 5.58×10−7 | hsa-let-7d-3p | 0.008 | hsa-let-7d-3p | 1.32×10−8 |
| hsa-miR-28-5p | 1.14 | 0.014 | hsa-miR-28-5p | 0.030 | hsa-miR-28-5p | 6.23×10−4 |
| hsa-miR-378a-3p | −1.50 | 9.93×10−5 | hsa-miR-378a-3p | 0.029 | hsa-miR-378a-3p | 9.94×10−6 |
| hsa-miR-26a-5p | −1.15 | 0.075 | hsa-miR-26a-5p | 0.046 | hsa-miR-26a-5p | 0.008 |
| hsa-miR-30b-5p | −1.19 | 0.036 | hsa-miR-30b-5p | 0.378 | hsa-miR-30b-5p | 0.026 |
| hsa-miR-140-3p | 1.13 | 0.018 | hsa-miR-140-3p | 0.389 | hsa-miR-140-3p | 0.018 |
| hsa-miR-30c-5p | −1.10 | 0.019 | hsa-miR-30c-5p | 0.019 | hsa-miR-30c-5p | 0.006 |
| hsa-miR-155-5p | 1.31 | 0.004 | hsa-miR-155-5p | 0.009 | hsa-miR-155-5p | 2.17×10−4 |
| hsa-miR-31-3p | −1.44 | 0.043 | hsa-miR-31-3p | 0.312 | hsa-miR-31-3p | 0.011 |
| hsa-miR-222-3p | 1.22 | 0.025 | hsa-miR-222-3p | 0.009 | hsa-miR-222-3p | 6.60×10−4 |
| hsa-miR-16-5p | 1.18 | 7.26×10−4 | hsa-miR-16-5p | 0.829 | hsa-miR-16-5p | 0.669 |
| hsa-miR-146a-5p | 1.30 | 0.017 | hsa-miR-146a-5p | 0.021 | hsa-miR-146a-5p | 0.001 |
| B cells | ||||||
| hsa-miR-30b-5p | −1.59 | 0.003 | hsa-miR-30b-5p | 0.012 | hsa-miR-30b-5p | 4.16×10−5 |
| hsa-miR-222-3p | 1.32 | 0.002 | hsa-miR-222-3p | 0.005 | hsa-miR-222-3p | 1.62×10−5 |
| hsa-miR-18a-3p | 1.28 | 0.027 | hsa-miR-18a-3p | 0.137 | hsa-miR-18a-3p | 0.613 |
| hsa-miR-19b-3p | −1.38 | 0.012 | hsa-miR-19b-3p | 0.028 | hsa-miR-19b-3p | 4.87×10−4 |
| hsa-miR-26a-5p | −1.37 | 0.013 | hsa-miR-26a-5p | 0.006 | hsa-miR-26a-5p | 9.85×10−5 |
| hsa-miR-20a-5p | −1.14 | 0.082 | hsa-miR-20a-5p | 0.019 | hsa-miR-20a-5p | 0.002 |
| hsa-miR-32-5p | −1.75 | 0.014 | hsa-miR-32-5p | 0.416 | hsa-miR-32-5p | 0.038 |
| hsa-let-7d-3p | −2.03 | 2.12×10−4 | hsa-let-7d-3p | 0.452 | hsa-let-7d-3p | 0.024 |
| hsa-miR-378a-3p | −1.29 | 0.062 | hsa-miR-378a-3p | 3.11×10−4 | hsa-miR-378a-3p | 5.49×10−6 |
| hsa-miR-146a-5p | −1.35 | 0.024 | hsa-miR-146a-5p | 0.157 | hsa-miR-146a-5p | 0.008 |
Differentially expressed miRNAs found in the discovery cohort and in the replication cohort are shown. MiRNAs in black depict those with a p value<0.05 and miRNAs in grey depict those with p value>0.05. Nominal p values without correction for multiple comparisons are shown.
miRNA, microRNA; pSS, primary Sjögren’s syndrome.