Cx43-interacting proteins identified by nanoLC-MS/MS
| Protein name | Accession no. | Score | Pep (95%) | % Cov |
|---|---|---|---|---|
| Microtubule | ||||
| Tubulin α-1A chain | Q71U36 | 25.68 | 16 | 55.9 |
| Tubulin α-1B chain | P68363 | 27.75 | 17 | 58.8 |
| Tubulin α-1C chain | Q9BQE3 | 23.28 | 13 | 41.4 |
| Tubulin β chain | P07437 | 27.04 | 19 | 53.8 |
| Tubulin β 2B chain | Q9BVA1 | 19.92 | 14 | 28.1 |
| Tubulin β 2A chain | Q13885 | 24.92 | 17 | 43.4 |
| Tubulin β 2C chain | P68371 | 21.86 | 15 | 47.4 |
| Actin filaments | ||||
| α—actinin-1 | P12814 | 12.9 | 9 | 30.5 |
| α—actinin-4 | O43707 | 17.34 | 11 | 27.9 |
| Actin, cytoplasmic-1 | P60709 | 36.68 | 28 | 70.1 |
| Actin, cytoplasmic-2 | P63261 | 9.88 | 16 | 57.3 |
| Others | ||||
| Vimentin | P08670 | 127.22 | 130 | 99.4 |
| Filamin-A | P21333 | 76.18 | 36 | 43.4 |
| Talin-1 | Q9Y490 | 14.17 | 8 | 29.8 |
| Vinculin | P18206 | 6.16 | 3 | 24.4 |
| Plectin | Q15149 | 6.68 | 4 | 25.2 |
| Erzin | P15311 | 2 | 1 | 5 |
| Myosin-9 | P35579 | 49.81 | 31 | 55 |
Identification parameters used with the ProteinPilot software are provided, including the protein name and accession number according to SwissProt databases, the score, the number of peptides at 95% confidence and the percentage of protein coverage (% Cov).
MS, mass spectrometry.