Differences in biomarker mRNA expression (relative units) and protein secretion (pg/ml) between cultured temporal artery sections from GCA patients and controls
| mRNA concentration (relative units) | Protein concentration (pg/ml) | |||||
|---|---|---|---|---|---|---|
| GCA biopsies | Control biopsies | p Value | GCA biopsies | Control biopsies | p Value | |
| IL-1β | 35.91±8.80 | 14.22±2.86 | 0.047 | 5.61±1.33 | −0.85±0.58 | 0.000 |
| IL-6 | 448.54±86.88 | 380.04±68.37 | 0.543 | 25583±9404 | 7805.4±2685 | 0.076 |
| TNFα | 4.70±2.26 | 6.69±1.24 | 0.420 | 21.25±5.09 | 8.42±2.59 | 0.053 |
| IFNγ | 0.805±0.257 | 0.012±0.011 | 0.010 | 7.75±2.41 | 7.75±2.41 | 0.764 |
| CCL-2/MCP-1 | 648.72±155.21 | 729.5±201.42 | 0.758 | 11268±1903 | 5850.91±4316.1 | 0.227 |
| CXCL-8/IL-8 | 2287.9±619.9 | 4346.5±1092.4 | 0.095 | 81403±25050 | 34778.2±18253.9 | 0.157 |
| CCL-3/MIP-1α | 86.31±16.9 | 20.16±.5.10 | 0.002 | 25.617±2.503 | 26.62±4.25 | 0.834 |
| CCL-4/MIP-1β | 28.21±6.13 | 5.36±1.13 | 0.003 | 12.61±3.1 | 6.15±1.3 | 0.087 |
| CCL-5/RANTES | 139.83±37.34 | 16.42±5.58 | 0.007 | 21.43±5.19 | 17.33±6.54 | 0.633 |
| MMP-2 | 2097.4±276.9 | 3450.9±1143.4 | 0.297 | 39125±11144 | 15250±8280 | 0.192 |
| MMP-9 | 1283.85±408.2 | 304.21±90.70 | 0.039 | 48913±10740 | 7825±3512.4 | 0.006 |
| TIMP-1 | 11813±3550 | 15126.8±5893.7 | 0.613 | Not done | Not done | – |
| TIMP-2 | 586.68±87.77 | 2798.1±1135.2 | 0.074 | Not done | Not done | – |
| COL I | 1545.6±284.61 | 1065.34±196.7 | 0.175 | Not done | Not done | – |
| COL III | 3674.4±637.07 | 3979.2±991.5 | 0.789 | Not done | Not done | – |
| PDGF A | 71.14±24.75 | 163.55±40.13 | 0.056 | Not done | Not done | – |
| PDGF B | 40.78±7.78 | 43.807±7.992 | 0.806 | Not done | Not done | – |
| PDGF AB | Not applicable | Not applicable | – | 23.375±3.245 | 41.50±15.34 | 0.325 |
Values in bold are statistically significant (p<0.05). mRNA expression was detected in the entire cohort of 28 GCA patients and 22 controls. CCL3/MIP-1α, CCL4/MIP-1β and CXCL8/IL-8 protein concentrations were detected by Luminex in 10 patients and six controls. The remaining proteins were detected by ELISA in the entire cohort.
COL, Collagen; GCA, giant-cell arteritis.