Differentially induced cytokine, chemokine and MMP secretion in RASF by adiponectin isoforms
| Protein name | Symbol | Ad isoform | Fold change | SEM | n | p Value |
|---|---|---|---|---|---|---|
| Chemokines | ||||||
| Chemokine (C-X-C motif) ligand 1 (CXCL1) | GRO-α | Native | 125.1 | 43.0 | 9 | 0.024 |
| HMW/MMW | 150.9 | 44.8 | 5 | 0.023 | ||
| Trimer | 19.0 | 12.5 | 5 | 0.223 | ||
| Globular | 75.8 | 26.4 | 5 | 0.027 | ||
| Chemokine (C-X-C motif) ligand 5 (CXCL5) | ENA-78 | Native | 22.5 | 6.2 | 13 | 0.005 |
| HMW/MMW | 29.5 | 5.4 | 8 | 0.001 | ||
| Trimer | 9.9 | 3.9 | 8 | 0.056 | ||
| Globular | 14.8 | 5.2 | 8 | 0.033 | ||
| Chemokine (C-X-C motif) ligand 6 (CXCL6) | GCP-2 | Native | 58.5 | 23.1 | 15 | 0.026 |
| HMW/MMW | 164.1 | 36.4 | 14 | 0.001 | ||
| Trimer | 22.8 | 6.1 | 14 | 0.003 | ||
| Globular | 110.3 | 35.8 | 14 | 0.009 | ||
| Chemokine (C-X-C motif) ligand 8 (CXCL8) | IL-8 | Native | 611.3 | 193.2 | 14 | 0.008 |
| HMW/MMW | 953.2 | 275.0 | 14 | 0.004 | ||
| Trimer | 135.6 | 50.8 | 14 | 0.020 | ||
| Globular | 570.5 | 223.1 | 14 | 0.024 | ||
| Chemokine (C-C motif) ligand 2 (CCL2) | MCP-1 | Native | 15.8 | 3.6 | 13 | 0.001 |
| HMW/MMW | 23.0 | 4.15 | 8 | 0.001 | ||
| Trimer | 17.2 | 7.9 | 8 | 0.081 | ||
| Globular | 18.5 | 6.9 | 8 | 0.040 | ||
| Chemokine (C-C motif) ligand 5 (CCL5) | RANTES | Native | 44.4 | 25.1 | 8 | 0.127 |
| HMW/MMW | 77.5 | 17.8 | 8 | 0.004 | ||
| Trimer | 10.0 | 7.8 | 8 | 0.381 | ||
| Globular | 145.3 | 35.6 | 8 | 0.005 | ||
| Cytokines | ||||||
| Activin A | INHBA | Native | 15.1 | 6.0 | 9 | 0.047 |
| HMW/MMW | 32.0 | 9.8 | 4 | 0.099 | ||
| Trimer | 7.3 | 2.2 | 4 | 0.065 | ||
| Globular | 2.8 | 1.2 | 4 | 0.212 | ||
| Interleukin 6 | IL-6 | Native | 31.2 | 9.7 | 12 | 0.010 |
| HMW/MMW | 57.0 | 16.9 | 12 | 0.007 | ||
| Trimer | 20.7 | 7.1 | 12 | 0.019 | ||
| Globular | 26.8 | 9.9 | 12 | 0.024 | ||
| Proteinases & Peptidases | ||||||
| Matrix metallopeptidase 1, propeptide | pro-MMP1 | Native | 11.9 | 8.1 | 13 | 0.206 |
| HMW/MMW | 24.4 | 15.9 | 13 | 0.014 | ||
| Trimer | 8.2 | 5.1 | 13 | 0.185 | ||
| Globular | 6.5 | 1.8 | 13 | 0.028 | ||
| Matrix metallopeptidase 3 | MMP3 | Native | 10.3 | 3.5 | 10 | 0.025 |
| HMW/MMW | 19.2 | 4.1 | 8 | 0.001 | ||
| Trimer | 4.3 | 1.5 | 8 | 0.063 | ||
| Globular | 12.6 | 3.8 | 8 | 0.014 | ||
| Matrix metallopeptidase 10 | MMP10 | Native | 4.4 | 1.1 | 11 | 0.009 |
| HMW/MMW | 11.6 | 2.1 | 11 | 0.002 | ||
| Trimer | 2.7 | 0.5 | 11 | 0.006 | ||
| Globular | 4.7 | 1.5 | 11 | 0.043 | ||
Multiple populations of RASF (n=4–15) were stimulated with different adiponectin isoforms. Secreted chemokines, cytokines and matrix metalloproteinases were quantified by immunoassays. Fold changes of secretion (as compared to an unstimulated control), biological variability indicated by the (SEM), number of populations analysed (n), and the p values are presented.
Ad, adiponectin; HMW, high molecular weight; MMP, matrix metalloproteinases; MMW, middle molecular weight; RASF, rheumatoid arthritis synovial fibroblasts.