Differentially induced chemokine mRNA expression in RASF by adiponectin isoforms
| Gene name | Symbol | Ad Isoform | Fold change | SEM | n | p Value |
|---|---|---|---|---|---|---|
| Chemokine (C-X-C motif) ligand 1 (CXCL1) | GRO-α | Native | 23.9 | 8.9 | 4 | 0.036 |
| HMW/MMW | 148.0 | 85.4 | 4 | 0.009 | ||
| Trimer | 14.1 | 8.1 | 4 | 0.021 | ||
| Globular | 206.8 | 134.9 | 4 | 0.009 | ||
| Chemokine (C-X-C motif) ligand 1 (CXCL2) | GRO-β | Native | 23.3 | 8.0 | 4 | 0.054 |
| HMW/MMW | 106.2 | 57.5 | 4 | 0.008 | ||
| Trimer | 9.3 | 4.5 | 4 | 0.021 | ||
| Globular | 121.1 | 68.8 | 4 | 0.008 | ||
| Chemokine (C-X-C motif) ligand 1 (CXCL3) | GRO-γ | Native | 66.3 | 50.8 | 4 | 0.020 |
| HMW/MMW | 77.7 | 36.7 | 4 | 0.005 | ||
| Trimer | 4.9 | 1.9 | 4 | 0.072 | ||
| Globular | 33.3 | 13.4 | 4 | 0.008 | ||
| Chemokine (C-X-C motif) ligand 5 (CXCL5) | ENA-78 | Native | 70.8 | 41.9 | 4 | 0.006 |
| HMW/MMW | 57.7 | 24.7 | 4 | 0.002 | ||
| Trimer | 3.8 | 0.6 | 4 | 0.002 | ||
| Globular | 22.2 | 8.4 | 4 | 0.054 | ||
| Chemokine (C-X-C motif) ligand 6 (CXCL6) | GCP-2 | Native | 112.0 | 45.7 | 4 | 0.019 |
| HMW/MMW | 584.6 | 327.7 | 4 | 0.003 | ||
| Trimer | 22.3 | 14.8 | 4 | 0.025 | ||
| Globular | 410.2 | 233.7 | 4 | 0.003 | ||
| Chemokine (C-C motif) ligand 2 (CCL2) | MCP-1 | Native | 7.2 | 1.9 | 4 | 0.008 |
| HMW/MMW | 11.9 | 3.0 | 4 | 0.005 | ||
| Trimer | 2.7 | 0.5 | 4 | 0.013 | ||
| Globular | 57.9 | 20.2 | 4 | 0.005 | ||
| Chemokine (C-C motif) ligand 7 (CCL7) | MCP-3 | Native | 16.3 | 9.1 | 4 | 0.015 |
| HMW/MMW | 17.8 | 7.9 | 4 | 0.007 | ||
| Trimer | 3.9 | 2.4 | 4 | 0.151 | ||
| Globular | 56.1 | 38.1 | 4 | 0.077 |
Multiple populations of RASF (n=4) were stimulated with different adiponectin isoforms. RNA was isolated, reverse transcribed to complementary DNA and quantified by real-time PCR. Fold changes in mRNA expression (as compared to an unstimulated control), biological variability indicated by the (SEM), number of populations analysed (n), and the p values are presented.
Ad, adiponectin; HMW, high molecular weight; MMW, middle molecular weight; RASF, rheumatoid arthritis synovial fibroblasts.