Affymetrix microarray results: differential gene induction in RASF by adiponectin isoforms
| Gene name | Gene symbol | Fold change native Ad | Fold change Ad HMW/MMW | Fold change Ad trimer | Fold change gAd |
|---|---|---|---|---|---|
| Chemokines | |||||
| Chemokine (C-C motif) ligand 2 (CCL2) | MCP-1 | 4.8 | 4.7 | 3.4 | 5.8 |
| Chemokine (C-C motif) ligand 5 (CCL5) | RANTES | 24.0 | 24.7 | 3.8 | 73.2 |
| Chemokine (C-C motif) ligand 7 (CCL7) | MCP-3 | 101.3 | 74.9 | 28.0 | 254.0 |
| Chemokine (C-C motif) ligand 8 (CCL8) | MCP-2 | 25.8 | 11.2 | 3.5 | 297.3 |
| Chemokine (C-C motif) ligand 20 (CCL20) | MIP-3α | 1424.0 | 1547.0 | 82.9 | 728.3 |
| Chemokine (C-X-C motif) ligand 1 (CXCL1) | GRO-α | 29.4 | 33.3 | 17.7 | 32.8 |
| Chemokine (C-X-C motif) ligand 2 (CXCL2) | GRO-β | 33.5 | 43.9 | 12.6 | 19.7 |
| Chemokine (C-X-C motif) ligand 3 (CXCL3) | GRO-γ | 99.6 | 234.0 | 34.6 | 60.2 |
| Chemokine (C-X-C motif) ligand 5 (CXCL5) | ENA-78 | 37.8 | 121.7 | 9.1 | 12.6 |
| Chemokine (C-X-C motif) ligand 6 (CXCL6) | GCP-2 | 5.5 | 5.6 | 3.7 | 4.1 |
| Chemokine (C-X-C motif) ligand 8 (CXCL8) | IL-8 | 43.4 | 50.5 | 28.2 | 47.6 |
| Chemokine (C-X-C motif) ligand 9 (CXCL9) | MIG | 9.3 | 36.7 | -3.1 | 48.9 |
| Chemokine (C-X-C motif) ligand 10 (CXCL10) | IP-10 | 161.0 | 78.6 | 2.1 | 1225.0 |
| Chemokine (C-X-C motif) ligand 11 (CXCL11) | I-TAC | 130.9 | 88.0 | -4.4 | 728.3 |
| Cytokines | |||||
| Interleukin 6 | IL-6 | 6.4 | 6.5 | 6.6 | 8.9 |
| Interleukin 11 | IL-11 | 24.3 | 441.0 | 157.4 | 2.3 |
| Other inflammatory molecules | |||||
| Prostaglandin E synthase | PTGES | 3.4 | 4.6 | 2.4 | 9.4 |
| Prostaglandin endoperoxide synthase 2/cyclooxygenase 2 | PTGS2/COX2 | 19.9 | 17.9 | 7.1 | 26.1 |
| Pre-B cell growth and B cell activation | |||||
| Bone marrow stromal cell antigen 2 | BST2 | 200.8 | 100.4 | 28.0 | 1091.0 |
| Receptors | |||||
| Interleukin 7 receptor | IL-7R | 5.6 | 6.9 | 3.0 | 8.9 |
| Interleukin 17 receptor B | IL-17RB | 2.6 | 2.1 | 1.0 | 2.8 |
| Proteinases and peptidases | |||||
| Matrix metallopeptidase 1 (interstitial collagenase) | MMP1 | 11.6 | 13.6 | 5.2 | 12.4 |
| Matrix metallopeptidase 3 (stromelysin 1 progelatinase) | MMP3 | 62.5 | 115.6 | 36.3 | 16.2 |
| Matrix metallopeptidase 10 (stromelysin 2) | MMP10 | 88.7 | 599.9 | 94.4 | 17.0 |
| Matrix metallopeptidase 12 (macrophage elastase) | MMP12 | 49.9 | 222.7 | 10.1 | 12.6 |
| Bone metabolism | |||||
| Stanniocalcin 1 | STC1 | 19.8 | 23.4 | 14.5 | 24.6 |
| Growth factors | |||||
| Fibroblast growth factor 10 | FGF10 | 5.0 | 5.6 | 1.0 | 3.9 |
RASF (n=1) were treated with adiponectin isoforms or left untreated (control). Affymetrix microarray analysis (GeneChip HG U133A) was performed as described. The results indicate that genes from several functional groups are differentially regulated in RASF by adiponectin isoforms. The cut-off value for fold changes was ≤2 (repression) or ≥2 (induction), respectively. Strongly repressed genes were low in number and of no known or well-defined function.
Ad, adiponectin; gAd, globular adiponectin; HMW, high molecular weight; MMW, middle molecular weight; RASF, rheumatoid arthritis synovial fibroblasts.