Table 1

Flow cytometry results normalised for background showing the specificity of MARB4 compared to MARB3

Peptide sequenceMARB3MARB4
No peptide11
GPKKIQKGK11
GRKKIQKGK2319
ARKKIQKGK201
VRKKIQKGK201
LRKKIQKGK211
IRKKIQKGK191
CRKKIQKGK241
MRKKIQKGK231
FRKKIQKGK211
YRKKIQKGK291
WRKKIQKGK281
PRKKIQKGK21
SRKKIQKGK151
TRKKIQKGK151
NRKKIQKGK161
QRKKIQKGK231
DRKKIQKGK101
ERKKIQKGK251
HRKKIQKGK251
KRKKIQKGK241
RRKKIQKGK161
GRKKIQKGK2838
ARKKIQKGK171
AGRKKIQKGK237
LGRKKIQKGK163
WGRKKIQKGK2516
AARKKIQKGK241
LARKKIQKGK281
WARKKIQKGK211
PAAPAAGRKKIQKGK184
PAAPAAARKKIQKGK291
  • Results of flow cytometric assays are shown here using BM36.1/B*27:05 cells that had been incubated with different peptides. All data are listed as multiples of background values obtained with isotype-matched control mAb and reflect mean values derived from at least three independent experiments. A non-binding control peptide exhibiting Pro at P2 was also employed. The cells were initially loaded with peptides that differed only in their N-terminal residue. The residues that underwent systematical exchanges are marked in bold letters. MARB3 is a peptide-dependent mAb with anti-Bw4 specificity that binds to HLA-B27 molecules as long as a peptide occupies the groove20. For MARB4, see text. The results for GRKKIQKGK and ARKKIQKGK are shown twice to allow for direct comparison with other peptides used in the same series of experiments. The original peptide ARKKIQKGK is derived from heat shock protein 57 of Chlamydia trachomatis32