Table 2 PCR primers/assays and siRNA used

Gene	Primer
MMP1	F 5′-AAGATGAAAGGTGGACCAACAATT-3′
	R 5′-CCAAGAGAATGGCCGAGTTC-3′
	Probe 5′-FAM-CAGAGAGTACAACTTACATCGTGTTGCGGCTC-TAMRA-3′
MMP13	F 5′-AAATTATGGAGGAGATGCCCATT-3′
	R 5′-TCCTTGGAGTGGTCAAGACCTAA-3′
	Probe 5′-FAM-CTACAACTTGTTTCTTGTTGCTGCGCATGA-TAMRA-3′
18S	F 5′-CGAATGGCTCATTAAATCAGTTATGG-3′
	R 5′-TATTAGCTCTAGAATTACCACAGTTATCC-3′
	Probe 5′-FAM-TCCTTTGGTCGCTCGCTCCTCTCCC-TAMRA-3′
MyD88	F 5′-CTTTTGGCAATCCTCCTCAA-3′
	R 5′-CTGCTCGAGCTGCTTACCA-3′
Trif	F 5′-CTCACCTGACCCCCTCCT-3′
	R 5′-AATTTCTGTTCCGATGATGATTC-3′
TLR3	F 5′-AGTTGTCATCGAATCAAATTAAAGAG-3′
	R 5′-AATCTTCCAATTGCGTGAAAA-3′
TLR1	Applied Biosystems: Assay Number: Hs00413978_m1
TLR2	Applied Biosystems: Assay Number: Hs00152932_m1
TLR4	Applied Biosystems: Assay Number: Hs00152939_m1
TLR6	Applied Biosystems: Assay Number: Hs00271977_s1
18S	Applied Biosystems: Assay Number: Hs99999901_s1

	ID/siRNA
MyD88	NM_002468/5′-GAAGAGAGCUGCUUAAACU-3′
	M-004769-00/5′-GAGAGUAGCUGUGUUUGAA-3′
	5′-CCAGAGCAAUUUAUACUUU-3′
	5′-GCACAUACAGACUCACAUA-3′
Trif	NM_014261/5′-GGACGAACACUCCCAGAUC-3′
	M-012833-00/5′-CCAUAGACCACUCAGCUUU-3′
	5′-GCCAGCAACUUGGAAAUCA-3′
	5′-GGAGCCACAUGUCAUUUGG-3′