Table 2 Antibody information
MoleculeIsotypeFluorophoreCloneCompany
CD19IgG1APC, FITCSJ25C1BD Biosciences
CD14IgG2bPerCP, PEMΦP9BD Biosciences
CD3IgG1FITC, PC-5UCHT1BD Biosciences
CD4IgG1PESK3BD Biosciences
BDCA2IgG1BiotinAC144Miltenyi Biotec*
BDCA3IgG1PEAD5-14H12Miltenyi Biotec*
DCIRIgG1κUnconjugated111F8Schering Plough†
DCIRIgG1Unconjugated216110 (MAB1748)R&D Systems
Goat anti-mouseF(ab’)2Alexa Fluor 647Molecular Probes
  • *Bergicsh, Gladbach, Germany. †Pateaux, France. APC, allophycocyanin; BDCA, blood dendritic cell antigen; DCIR, dendritic cell immunoreceptor; FITC, fluorescein isothiocyanate; PC-5, proprotein convertase 5; PE, phycoerythrin; PerCP, peridinin–chlorophyll–protein complex.