Table 2 Antibodies used for immunohistochemical stainings*
Primary antibodyIsotypeSupplier
Cell marker:
CD3SK7Mouse IgG1Becton-Dickinson, San Jose, California, USA
CD4SK3Mouse IgG1Becton-Dickinson, San Jose, California, USA
CD8SK1Mouse IgG1Becton-Dickinson, San Jose, California, USA
Ki67Ki-S55Mouse IgG1Dakopatts A/S, Glostrup, Denmark
CD68EMB111Mouse IgG1Dakopatts A/S, Glostrup, Denmark
CD163Ber-MAC31Mouse IgG1Dakopatts A/S, Glostrup, Denmark
MHC and cytokines:
MHC class IW6/325Mouse IgG2ADakopatts A/S, Glostrup, Denmark
MHC class IIL243Mouse IgG2ABecton-Dickinson, San Jose, California, USA
IL1α1277-89-7Mouse IgG1Immunokontakt, Bioggo, Switzerland
IL1β2D8 combined with 1437-96-5Mouse IgG1Immunokontakt, Bioggo, Switzerland
IL6MQ2-6A3Rat IgG2ABD, PharMingen, San Diego, California, USA
TNF2C8Mouse IgG1Biodesign, Saco, ME
TNFMab1 combined with Mab11Mouse IgG1BD, PharMingen, San Diego, California, USA
HMGB-1556528Rabbit IgGBD, PharMingen, San Diego, California, USA
MxAM143Mouse IgG2AKind gift from the Univeritäts Klinikum of Freiburg
IFNγDIK-1Mouse IgG1Mabtech, Sweden
MACaE111Mouse IgG2ADakopatts A/S, Glostrup, Denmark
Vessel marker:
CD31EN4Mouse IgG1Sanbio, Bio-Zac, Uden, The Netherlands
Secondary antibodies:Biotinylated F(ab′)2 –fragmented goat anti-mouse IgG1Catalog Laboratories, Burlingame, California, USA
Biotinylated horse anti-mouse IgG1Vector Laboratories, Burlingame, California, USA
Biotinylated AB2, ChemMate Detection Kit, Code No. K5001DakoCytomation A/S, Glostrup, Denmark
Biotinylated goat anti-rabbit IgGVector Laboratories, Burlingame, California, USA
Negative controls:Mouse irrelevant IgG1, X 0931Dakopatts A/S, Glostrup, Denmark
Mouse irrelevant IgG2A, X 0943Dakopatts A/S, Glostrup, Denmark
Mouse irrelevant IgG2A, DAK-G05Dakopatts A/S, Glostrup, Denmark
Irrelevant rabbit serumDakopatts A/S, Glostrup, Denmark
  • *CD3, reacts with epsilon chain of the CD3 antigen/T cell antigen receptor (TCR) complex; CD4, marker for CD4+ helper/inducer T lymphocytes; CD8, marker for CD8+ suppressor/cytotoxic T lymphocytes; Ki67, marker for proliferating cells expressing Ki-67 antigen; CD68, macrophage marker; CD163, marker for stimulated monocytes and resident tissue macrophages. CD31, endothelial cell marker.

  • HMGB-1, high mobility group box chromosomal protein 1; IFN, interferon; IL, interleukin; MAC, membrane attack complex; MHC, major histocompatibility complex; MxA, myxovirus resistance protein A (a marker for IFNα); TNF, tumour necrosis factor (clone 2C8 is a non-neutralising antibody and Mab1 combined with Mab11 are neutralising antibodies).