Table 1 Determination of affinity of nucleosomes and antibodies for glomerular collagen IV and laminin by surface plasmon resonance analysis

Ligand on chip	Analyte	k_a (103/M/s)	k_d (10⁻⁴/s)	K_D (10⁻⁸ M)	χ² (RU2)
Collagen IV	Nuc*	12.1	3.896	3.201	104
	SN†	7.553	1.293	1.712	30.0
	Anticollagen IV	8.116	4.157	5.122	241
Laminin	Nuc	44.56	4.306	0.967	29.0
	SN	12.51	1.285	1.027	23.2
	Antilaminin	6.086	0.521	0.855	4.95×103
Collagen IV/laminin	Nuc	9.789	1.775	1.813	8.83
	Antilaminin	7.991	0.448	0.561	1.52×104
	Anticollagen IV	12.89	2.151	1.669	71.1

*Nuc, eukaryotic, non-stripped nucleosomes generated by micrococcal nuclease digestion. †SN, stripped nucleosomes.