Table 2

 The VH PCR oligonucleotide primers

Amino acid positions in parentheses.
(1) 5′ primers
VH15′CTACGTCGAC CCTCAGTGAA GGTYTCCTGC AAGGC(16–24)
VH25′GCACGTCGAC GTCCTGCGCT GGTGAAASCC ACACA(16–24)
VH35′GTACGTCGAC GGGGTCCCTG AGCTCTCCTG TGCAG(15–24)
VH45′CGTCGTCGAC CCTGTCCCTC ACCTGCRCTG TC(16–24)
VH55′CGACGTCGAC AAAAAGCCCG GGGAGTCTCT GARGA(12–20)
VH65′CGTCGTCGAC CTGTGCCATC TCCGGGGACA GTG(21–29)
(2) External 3′ primers
JH Intron 1,2–4,55′GACTCACCTG AGGAGACGGT GACC
JH Intron 3,65′TCTTGCCTGA GGAGACGGTG ACCRT
(3) Internal 3′ primers
JH15′CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC(101–109)
JH25′CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC(101–109)
JH35′CGTCGTCGAC ATTGTCCCTT GGCCCCAGAC ATCA(100–108)
JH45′CGTCGTCAAC CACGGTTCCT TGGCCCCAGT AG(101–109)
JH55′CGTCGTCGAC GTGACCAGGG TTCCTTGGCC CCAGG(102–110)
JH65′CGTCGTCGAC GTGGTCCCTT GCCCCCAGAC GTCC(100–108)