The VH PCR oligonucleotide primers
| Amino acid positions in parentheses. | ||||
| (1) 5′ primers | ||||
| VH1 | 5′CTACGTCGAC CCTCAGTGAA GGTYTCCTGC AAGGC | (16–24) | ||
| VH2 | 5′GCACGTCGAC GTCCTGCGCT GGTGAAASCC ACACA | (16–24) | ||
| VH3 | 5′GTACGTCGAC GGGGTCCCTG AGCTCTCCTG TGCAG | (15–24) | ||
| VH4 | 5′CGTCGTCGAC CCTGTCCCTC ACCTGCRCTG TC | (16–24) | ||
| VH5 | 5′CGACGTCGAC AAAAAGCCCG GGGAGTCTCT GARGA | (12–20) | ||
| VH6 | 5′CGTCGTCGAC CTGTGCCATC TCCGGGGACA GTG | (21–29) | ||
| (2) External 3′ primers | ||||
| JH Intron 1,2–4,5 | 5′GACTCACCTG AGGAGACGGT GACC | |||
| JH Intron 3,6 | 5′TCTTGCCTGA GGAGACGGTG ACCRT | |||
| (3) Internal 3′ primers | ||||
| JH1 | 5′CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC | (101–109) | ||
| JH2 | 5′CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC | (101–109) | ||
| JH3 | 5′CGTCGTCGAC ATTGTCCCTT GGCCCCAGAC ATCA | (100–108) | ||
| JH4 | 5′CGTCGTCAAC CACGGTTCCT TGGCCCCAGT AG | (101–109) | ||
| JH5 | 5′CGTCGTCGAC GTGACCAGGG TTCCTTGGCC CCAGG | (102–110) | ||
| JH6 | 5′CGTCGTCGAC GTGGTCCCTT GCCCCCAGAC GTCC | (100–108) | ||