Effects of caspase inhibitors on viability in OKA treated cultured OA chondrocytes
| Stimulus | 48 Hours | 72 Hours |
|---|---|---|
| Confluent chondrocytic cells were preincubated for 2 hours in the absence or presence of caspase inhibitors at 100 μmol/l (Z-VAD-FMK, a caspase general inhibitor or Z-DEVD-FMK, a caspase 3 inhibitor) both in basal conditions or with OKA (50 nmol/l) for an additional 48 or 72 hours. The data are expressed as percentages with respect to basal conditions, and are the mean (SE) of six independent experiments in triplicate.*p⩽0.001 v untreated chondrocytes; †p⩽0.001 v OKA; †p⩽0.05 v OKA. | ||
| Basal | 100 | 100 |
| Basal +Z-VAD-FMK | 93 (4.4) | 94 (5.7) |
| Basal +Z-DEVD-FMK | 97 (2.8) | 96 (5.2) |
| OKA | 60 (2.5)* | 43 (1.8)* |
| OKA+Z-VAD-FMK | 87 (5.0)† | 78 (4.5)† |
| OKA+Z-DEVD-FMK | 73 (4.2)‡ | 58 (2.5)‡ |